{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T13:10:33Z","timestamp":1778937033489,"version":"3.51.4"},"reference-count":77,"publisher":"Wiley","issue":"s1","license":[{"start":{"date-parts":[[2007,10,24]],"date-time":"2007-10-24T00:00:00Z","timestamp":1193184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2007,10,24]],"date-time":"2007-10-24T00:00:00Z","timestamp":1193184000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objective\u2002<\/jats:bold>\n                    To identify canine\n                    <jats:italic>myocilin<\/jats:italic>\n                    cDNA and compare its sequence in glaucomatous and nonglaucomatous Shiba Inu dogs with closed and open iridocorneal angles (ICAs).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Procedures\u2002<\/jats:bold>\n                    Total RNA was extracted from the ciliary body of the eyes of a healthy Beagle, and the canine\n                    <jats:italic>myocilin<\/jats:italic>\n                    gene was cloned and sequenced. Of the Shiba Inu dogs tested, five were glaucomatous with closed ICA, three were nonglaucomatous with open ICA, and two were nonglaucomatous with closed ICA. The genomic DNA of these dogs was extracted from peripheral blood leukocytes. The exons of the canine\n                    <jats:italic>myocilin<\/jats:italic>\n                    gene were amplified using the polymerase chain reaction (PCR), and sequenced. The frequency of mutation in canine\n                    <jats:italic>myocilin<\/jats:italic>\n                    DNA was verified in these dogs by using the\n                    <jats:italic>myocilin<\/jats:italic>\n                    cDNA of a Beagle.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results\u2002<\/jats:bold>\n                    The canine\n                    <jats:italic>myocilin<\/jats:italic>\n                    cDNA was 1452\u00a0bp long and contained the entire open reading frame encoding 483 amino acids. A leucine zipper\u2010like motif and olfactomedin\u2010like domain were conserved in the amino acid residues. The presence of sequence variants in the genomic DNA of Shiba Inu dogs was independent of the occurrence of glaucoma and ICA grading.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions\u2002<\/jats:bold>\n                    <jats:italic>Myocilin<\/jats:italic>\n                    RNA was detected in the ciliary body and trabecular meshwork (TM) of a Beagle. The\n                    <jats:italic>myocilin<\/jats:italic>\n                    sequence of Shiba Inu dogs suggests that\n                    <jats:italic>myocilin<\/jats:italic>\n                    mutations are unlikely to play a significant role in the pathogenesis of primary closed\u2010angle glaucoma in this breed. However, several mutations in the\n                    <jats:italic>myocilin<\/jats:italic>\n                    gene in exon 1 of Shiba Inu dogs may predispose them to an obstruction in the anterior aqueous outflow.\n                  <\/jats:p>","DOI":"10.1111\/j.1463-5224.2007.00530.x","type":"journal-article","created":{"date-parts":[[2007,10,27]],"date-time":"2007-10-27T05:04:31Z","timestamp":1193461471000},"page":"53-62","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Cloning of canine\n                    <i>myocilin<\/i>\n                    cDNA and molecular analysis of the\n                    <i>myocilin<\/i>\n                    gene in Shiba Inu dogs"],"prefix":"10.1111","volume":"10","author":[{"given":"Kumiko","family":"Kato","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuo","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Matsunaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryohei","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Ogawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,10,24]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.80.5.389"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.1993.tb04989.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.85.11.1277"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2006.00438.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1463-5224.2002.00213.x"},{"key":"e_1_2_6_7_2","first-page":"701","volume-title":"Veterinary 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