{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:36:07Z","timestamp":1767854167261,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"s1","license":[{"start":{"date-parts":[[2011,9,16]],"date-time":"2011-09-16T00:00:00Z","timestamp":1316131200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objective\u2002<\/jats:bold>To determine the presence of chlamydial species including recently described chlamydial agents as well as the human pathogen<jats:italic>Chlamydophila pneumoniae<\/jats:italic>in feline conjunctivitis.<\/jats:p><jats:p><jats:bold>Animal studied\u2002<\/jats:bold>Twenty five cats without and 49 cats with conjunctivitis were tested for chlamydia using a<jats:italic>Chlamydiaceae<\/jats:italic>real time (RT) PCR (targeting the 23S rRNA gene sequence), a<jats:italic>Chlamydiales<\/jats:italic>PCR (targeting the 16S rRNA gene sequence), and cell culture. The PCR products of all positive samples were sequenced and subsequently analyzed using a basic local alignment search tool search.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold><jats:italic>Chlamydiaceae<\/jats:italic>RT PCR and subsequent sequence analyses identified<jats:italic>C<\/jats:italic>.<jats:italic>pneumoniae<\/jats:italic>in five cats in the conjunctivitis group. The presence of<jats:italic>Chlamydophila felis<\/jats:italic>was shown in two cats with conjunctivitis. Chlamydiae related to uncultured members of<jats:italic>Chlamydiales<\/jats:italic>were detected in three conjunctivitis cases and in one cat without clinical symptoms.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold>This study detects for the first time, the known human pathogen<jats:italic>C<\/jats:italic>.<jats:italic>pneumoniae<\/jats:italic>in feline conjunctivitis cases using<jats:italic>Chlamydiaceae<\/jats:italic>RT PCR and sequence analyses.<\/jats:p>","DOI":"10.1111\/j.1463-5224.2011.00919.x","type":"journal-article","created":{"date-parts":[[2011,9,16]],"date-time":"2011-09-16T19:32:17Z","timestamp":1316201537000},"page":"67-74","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Detection of<i>Chlamydophila pneumoniae<\/i>in cats with conjunctivitis"],"prefix":"10.1111","volume":"14","author":[{"given":"Christina","family":"Sibitz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth C.","family":"Rudnay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leila","family":"Wabnegger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim","family":"Spergser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Apfalter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Nell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,9,16]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"541","article-title":"Experimentally induced feline chlamydial infection (feline pneumonitis)","volume":"39","author":"Hoover EA","year":"1978","journal-title":"American Journal of Veterinary Research"},{"key":"e_1_2_5_3_2","first-page":"304","volume-title":"Feline Medicine and Therapeutics","author":"Wills JM","year":"1985"},{"key":"e_1_2_5_4_2","first-page":"1095","volume-title":"Veterinary Ophtalmology","author":"Stiles J","year":"2007"},{"key":"e_1_2_5_5_2","doi-asserted-by":"crossref","first-page":"2314","DOI":"10.2460\/ajvr.1984.45.11.2314","article-title":"Pathogenesis of feline gastric chlamydial infection","volume":"45","author":"Gaillard ET","year":"1984","journal-title":"American Journal of Veterinary Research"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.134.15.365"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.96.2499.475"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-2-415"},{"key":"e_1_2_5_9_2","first-page":"932","article-title":"Microbiological and immunologic aspects of feline pneumonitis","volume":"158","author":"Cello RM","year":"1971","journal-title":"Journal of the American Veterinary Medical Association"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.1988.tb02293.x"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.1999.tb02171.x"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(97)00185-5"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.64.1165"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.1995.35890"},{"key":"e_1_2_5_15_2","first-page":"165","article-title":"Demonstration of Chlamydia psittaci in feline conjunctivitis cases in Belgium","volume":"58","author":"Hanselaer JA","year":"1989","journal-title":"Vlaams Diergeneeskd Tijdschr"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.63.937"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.68.6.643"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2003.tb02517.x"},{"key":"e_1_2_5_19_2","first-page":"231","article-title":"A survey of the conjunctival flora of clinically normal cats and cats with conjunctivitis","volume":"21","author":"Shewen PE","year":"1980","journal-title":"Canadian Veterinary Journal"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.1999.tb02172.x"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(02)01369-4"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.2.6147.1264"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-4453(01)90845-X"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45920-0"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1969.00990020587003"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.42.3.349"},{"key":"e_1_2_5_27_2","first-page":"289","article-title":"Case report. 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