{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:03:30Z","timestamp":1761764610312,"version":"build-2065373602"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:sec>\n                    <jats:title>OBJECTIVE<\/jats:title>\n                    <jats:p>To study the effect of 3\u2003weeks of partial bladder outlet obstruction (BOO), compared to a sham operation, on the cholinergic and purinergic components of detrusor contractile responses to agonists and to electrical field stimulation (EFS); the expression of P2X receptor subtypes was also examined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>MATERIALS AND METHODS<\/jats:title>\n                    <jats:p>Partial BOO was induced in female Sprague\u2013Dawley rats by surgically applying a jeweller's silver \u2018jump\u2019 ring around the urethra, such that the urethra was constricted but not closed. Sham\u2010operated female rats underwent an identical procedure without placement of a ring.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      After 3\u2003weeks of partial BOO the rat bladders became significantly hypertrophied, doubling in weight. Spontaneous activity was markedly increased, but the contractile response to a single bolus of KCl (120\u2003m\n                      <jats:sc>m<\/jats:sc>\n                      ) was unaltered. The neurogenic\u2010induced contractile responses of strips of detrusor from obstructed bladders were significantly greater than those from sham\u2010operated bladders, and the responses of strips of detrusor from obstructed bladders to EFS showed a significantly greater atropine\u2010sensitive component than sham\u2010operated detrusor. However, the response of detrusor strips to EFS that was susceptible to desensitization by \u03b1,\u03b2\u2010methylene ATP was not significantly changed in obstructed bladders. The sensitivity of the strips from obstructed bladders to carbachol, ATP and \u03b2,\u03b3\u2010methylene ATP was less than in sham\u2010operated detrusor. Immunohistochemical studies showed no difference in the P2X receptor subtypes expressed on detrusor smooth muscle from obstructed and sham\u2010operated rats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>In the rat, after moderate bladder hypertrophy, the atropine\u2010sensitive component was significantly up\u2010regulated, but the ATP\u2010sensitive component was marginally reduced, although not significantly. These results suggest that up\u2010regulation of the P2X component of bladder contraction seen in humans with bladder instability, and in other species models of BOO, is not mirrored in the rat, or occurs later in the pathological process of bladder hypertrophy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/j.1464-410x.2006.06010.x","type":"journal-article","created":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T05:05:35Z","timestamp":1132635935000},"page":"372-378","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Alterations in purinergic and cholinergic components of contractile responses of isolated detrusor contraction in a rat model of partial bladder outlet obstruction"],"prefix":"10.1111","volume":"97","author":[{"given":"FREDERICK C.L.","family":"BANKS","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GILLIAN 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