{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T07:14:30Z","timestamp":1781075670844,"version":"3.54.1"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,4]],"date-time":"2006-07-04T00:00:00Z","timestamp":1151971200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:sec>\n                    <jats:title>OBJECTIVE<\/jats:title>\n                    <jats:p>To investigate whether variable coding sequence protein A1 (Vcsa1) is down\u2010regulated in rat models of diabetes and ageing, and to investigate the role of Vcsa1 in erectile function, as Vcsa1 is the most down\u2010regulated gene in the corpora of a rat model of neurogenic erectile dysfunction (ED).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>MATERIALS AND METHODS<\/jats:title>\n                    <jats:p>Quantitative reverse\u2010transcriptase polymerase\u2010chain reaction was used to determine Vcsa1 expression in the corpora of rats in three models of ED, i.e. streptozotocin\u2010induced diabetes, retired breeder (old), and neurogenic (bilaterally ligated cavernosal nerves), and in control rats. To confirm a physiological role of Vcsa1 in erectile function, we carried out gene transfer studies using a plasmid in which Vcsa1 was expressed from a cytomegalovirus promoter (pVAX\u2010Vcsa1). This plasmid was injected intracorporally into old rats, and the effect on physiology of corporal tissue was analysed by intracorporal\/blood pressure (ICP\/BP) measurement and histological analysis, and compared with the effects of a positive control plasmid (pVAX\u2010hSlo, which we previously reported to restore erectile function in diabetic and ageing rats) and a negative control plasmid (pVAX).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>In each rat model of ED there was a significant down\u2010regulation of the Vcsa1 transcript of at least 10\u2010fold in corporal tissue. Remarkably, intracorporal injection with 80\u2003\u00b5g pVAX\u2010Vcsa1 caused priapism, as indicated by visible prolonged erection, histological appearance, and elevated resting ICP\/BP. Lower doses of pVAX\u2010Vcsa1 (5 and 25\u2003\u00b5g) increased ICP\/BP over that in untreated controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>These results show that Vcsa1 has a role in erectile function and might be a molecular marker for organic ED. The role of Vcsa1 in erectile function suggests that it could represent a novel therapeutic target for treating ED.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/j.1464-410x.2006.06247.x","type":"journal-article","created":{"date-parts":[[2006,7,6]],"date-time":"2006-07-06T21:41:38Z","timestamp":1152222098000},"page":"396-401","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Variable coding sequence protein A1 as a marker for erectile dysfunction"],"prefix":"10.1111","volume":"98","author":[{"given":"YUEHONG","family":"TONG","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MOSES","family":"TAR","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FELIX","family":"DAVELMAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GEORGE","family":"CHRIST","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ARNOLD","family":"MELMAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KELVIN P.","family":"DAVIES","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,4]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000060882.75475.5a"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb18556.x"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2004.06.012"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1431850100"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1997.273.4.R1309"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/41.11.8409372"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijir.3900396"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1385\/ENDO:23:2-3:087"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000165389.73148.d1"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000075362.08363.a4"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00112.2005"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.275.2.H600"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000063375.12512.6e"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2005.05.005"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00792.2003"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04226.x"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.142.10.4550"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijir.3900907"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a021689"}],"container-title":["BJU International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1464-410X.2006.06247.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1464-410X.2006.06247.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T17:23:59Z","timestamp":1761758639000},"score":1,"resource":{"primary":{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/10.1111\/j.1464-410X.2006.06247.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7,4]]},"references-count":19,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,8]]}},"alternative-id":["10.1111\/j.1464-410X.2006.06247.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1464-410x.2006.06247.x","archive":["Portico"],"relation":{},"ISSN":["1464-4096","1464-410X"],"issn-type":[{"value":"1464-4096","type":"print"},{"value":"1464-410X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,7,4]]},"assertion":[{"value":"2006-07-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}