{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T08:37:50Z","timestamp":1783759070613,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,5,21]],"date-time":"2005-05-21T00:00:00Z","timestamp":1116633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabetic Medicine"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aims\u2003<\/jats:bold> Mutations in the serine protease inhibitor (SPINK1) gene have been associated with all forms of chronic pancreatitis. Recently, an association of SPINK1 mutations with early\u2010onset Type\u00a02 diabetes mellitus has been reported in patients from Bangladesh. Therefore, we determined the frequency of SPINK1 N34S mutations in patients with Type\u00a02 diabetes mellitus from the USA.<\/jats:p><jats:p><jats:bold>Methods\u2003<\/jats:bold> The study population of Hispanic and non\u2010Hispanic white people consisted of 387 patients with Type\u00a02 diabetes and familial clustering of the disease, 232 family members without diabetes, 259 patients with Type\u00a02 diabetes without a family history, and 302 ethnically matched healthy controls as part of the San Luis Valley Diabetes Study. We performed linkage\u2010 and association\u2010analysis in 82 multiplex families with Type\u00a02 diabetes mellitus.<\/jats:p><jats:p><jats:bold>Results\u2003<\/jats:bold> No significant linkage or allele sharing was detected between Type\u00a02 diabetes mellitus and the SPINK1 locus. The frequency of the N34S mutation was determined by fluorescence polarization and was similar between patients (<jats:italic>n<\/jats:italic>\u00a0=\u00a014\/387 patients with familial clustering; <jats:italic>n<\/jats:italic>\u00a0=\u00a02\/259 patients without family history) and controls (<jats:italic>n<\/jats:italic>\u00a0=\u00a05\/232 family members without diabetes; <jats:italic>n<\/jats:italic>\u00a0=\u00a010\/302 individuals). Variables such as ethnicity, age of diabetes onset and percentage of individuals with impaired glucose tolerance did not differ significantly between carriers and homozygous normal individuals.<\/jats:p><jats:p><jats:bold>Conclusion\u2003<\/jats:bold> The SPINK1 N34S mutation appears not to predispose Hispanic or non\u2010Hispanic white people from the USA to the development of Type\u00a02 diabetes mellitus.<\/jats:p>","DOI":"10.1111\/j.1464-5491.2005.01513.x","type":"journal-article","created":{"date-parts":[[2005,5,21]],"date-time":"2005-05-21T17:24:25Z","timestamp":1116696265000},"page":"744-748","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The SPINK1 N34S mutation is not associated with Type\u00a02 diabetes mellitus in a population of the USA"],"prefix":"10.1111","volume":"22","author":[{"given":"A.","family":"Schneider","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. C.","family":"Lawrence","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. M.","family":"Barmada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Norris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. F.","family":"Hamman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. A.","family":"Marshall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. E.","family":"Ferrell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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