{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:12:46Z","timestamp":1791180766537,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2010,6,8]],"date-time":"2010-06-08T00:00:00Z","timestamp":1275955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabetic Medicine"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>Diabet. Med. 27, 1178\u20131187 (2010)<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      <jats:bold>Aims\u2002<\/jats:bold>\n                      Beradinelli\u2013Seip congenital generalized lipodystrophy is a rare autosomal recessive disorder characterized by near\u2010complete absence of adipose tissue, Herculean appearance, insulin resistance, hypoleptinaemia and diabetes mellitus. The aim of this study was to investigate the\n                      <jats:italic>in vitro<\/jats:italic>\n                      effects of pioglitazone on the expression of genes involved in adipogenesis in fibroblasts from a patient with this condition due to a seipin mutation.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Methods\u2002<\/jats:bold>\n                      Primary cultures of fibroblasts from the skin of the patient were obtained. Fibroblasts were treated with classic adipose differentiation medium, with and without pioglitazone. Several adipogenes were evaluated by real\u2010time reverse transcriptase\u2010polymerase chain reaction and western blotting. Intracellular localization of prelamin\u2003A was studied by immunofluorescence microscopy.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Results\u2002<\/jats:bold>\n                      The expression of the adipogenic genes\n                      <jats:italic>PPARG<\/jats:italic>\n                      ,\n                      <jats:italic>LPL<\/jats:italic>\n                      ,\n                      <jats:italic>LEP<\/jats:italic>\n                      and\n                      <jats:italic>SLC2A4<\/jats:italic>\n                      was reduced in lipodystrophic fibroblasts, while treatment with pioglitazone increased the expression of these genes. Moreover, and unexpectedly, we found an accumulation of farnesylated prelamin\u2003A in lipodystrophic fibroblasts.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Conclusions\u2002<\/jats:bold>\n                      The process of adipocyte differentiation is compromised in patients with Beradinelli\u2010Seip congenital lipodystrophy owing to diminished expression of the regulatory genes involved, which pioglitazone treatment partially rescues. Prelamin\u2003A accumulation establishes a link with other types of familial lipodystrophies, as familial partial lipodystrophy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/j.1464-5491.2010.03052.x","type":"journal-article","created":{"date-parts":[[2010,6,8]],"date-time":"2010-06-08T07:54:53Z","timestamp":1275983693000},"page":"1178-1187","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Reduced adipogenic gene expression in fibroblasts from a patient with type 2 congenital generalized lipodystrophy"],"prefix":"10.1111","volume":"27","author":[{"given":"B.","family":"Victoria","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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