{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T06:53:02Z","timestamp":1760079182834},"reference-count":7,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intern J of Cosmetic Sci"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Synopsis<\/jats:title><jats:p>Females in South East Asia (Thailand, Indonesia and the Philippines) show concern about dark areas of skin which develop in their underarms, but little is known about the features differentiating pale and hyperpigmented axillary skin in the general population. To investigate this, a histology study was undertaken in the Philippines to define the aetiology of underarm darkening, which is postulated to be a mild form of postinflammatory hyperpigmentation (PIHP). Punch biopsies were taken from dark and light axillary skin sites of 20 female subjects, of whom seven had hyperpigmented underarms, based on an instrumental (Mexameter MX\u201018, Courage and Khazaka Electronic GmbH, Cologne, Germany) measure, and 13 had not. Histological and immunohistochemical analyses were undertaken using a range of stains and antibodies, including haematoxylin\u2010eosin for general histopa\u2010thology, Masson\u2010Fontana for melanin, anti\u2010CD68 for monocytes and macrophages, Van Gieson's technique for fibrosis, anti\u2010proliferating cell nuclear antigen for cell mitosis, and the melanocyte\u2010specific immunostains, anti\u2010tyrosinase and anti\u2010tyrosinase\u2010related protein 1. In most cases, dark skin sites from hyperpigmented panellists had increased intensity of Masson\u2010Fontana, anti\u2010tyrosinase and\/or anti\u2010TRP1 staining, indicative of melanocyte stimulation and increased melanin production. Furthermore, hair plucking emerged as a key stimulus to increased pigmentation. The trauma of hair plucking slightly increased the number of infiltrating mononuclear cells and macrophages that ingested melanosomes leaking from the damaged epidermis, more so in the skin of hyperpigmented panellists; this, in turn, potentially increases pigmentation. However, cell infiltration was focal, mainly near the plucked follicles, and not indicative of diffuse inflammation. The results from this study support the hypothesis that axillary darkening is mild PIHP, characterized by increased epidermal melanin, following stimulation or mild irritation of skin, with hair plucking as a key factor in this process.<\/jats:p>","DOI":"10.1111\/j.1467-2494.2006.00328.x","type":"journal-article","created":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T13:41:05Z","timestamp":1153143665000},"page":"247-253","source":"Crossref","is-referenced-by-count":12,"title":["Histological evaluation of hyperpigmentation on female Filipino axillary skin<sup>1<\/sup>"],"prefix":"10.1111","volume":"28","author":[{"given":"A. G.","family":"James","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. E.","family":"Pople","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. E.","family":"Parish","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. E.","family":"Moore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Dunbar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1786","volume-title":"Textbook of dermatology","author":"Bleehen S.S.","year":"1998"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2004.02267.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1950.30.1.91"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0216com"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2004.04.045"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.1994.tb00050.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.dad.0000154419.18653.2e"}],"container-title":["International Journal of Cosmetic Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1467-2494.2006.00328.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1467-2494.2006.00328.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T17:53:25Z","timestamp":1696960405000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1467-2494.2006.00328.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7,17]]},"references-count":7,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,8]]}},"alternative-id":["10.1111\/j.1467-2494.2006.00328.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1467-2494.2006.00328.x","archive":["Portico"],"relation":{},"ISSN":["0142-5463","1468-2494"],"issn-type":[{"value":"0142-5463","type":"print"},{"value":"1468-2494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,7,17]]}}}