{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:17:16Z","timestamp":1791091036739,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,1,25]],"date-time":"2007-01-25T00:00:00Z","timestamp":1169683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Eastern cottonwood (<jats:italic>Populus deltoides<\/jats:italic> Bartr. ex Marsh.) trees were engineered to express <jats:italic>merA<\/jats:italic> (mercuric ion reductase) and <jats:italic>merB<\/jats:italic> (organomercury lyase) transgenes in order to be used for the phytoremediation of mercury\u2010contaminated soils. Earlier studies with <jats:italic>Arabidopsis thaliana<\/jats:italic> and <jats:italic>Nicotiana tabacum<\/jats:italic> showed that this gene combination resulted in more efficient detoxification of organomercurial compounds than did <jats:italic>merB<\/jats:italic> alone, but neither species is optimal for long\u2010term field applications. Leaf discs from <jats:italic>in vitro<\/jats:italic>\u2010grown <jats:italic>merA, nptII<\/jats:italic> (neomycin phosphotransferase) transgenic cottonwood plantlets were inoculated with <jats:italic>Agrobacterium tumefaciens<\/jats:italic> strain C58 carrying the <jats:italic>merB<\/jats:italic> and hygromycin resistance (<jats:italic>hptII<\/jats:italic>) genes. Polymerase chain reaction of shoots regenerated from the leaf discs under selection indicated an overall transformation frequency of 20%. Western blotting of leaves showed that MerA and MerB proteins were produced. <jats:italic>In vitro<\/jats:italic>\u2010grown <jats:italic>merA<\/jats:italic>\/<jats:italic>merB<\/jats:italic> plants were highly resistant to phenylmercuric acetate, and detoxified organic mercury compounds two to three times more rapidly than did controls, as shown by mercury volatilization assay. This indicates that these cottonwood trees are reasonable candidates for the remediation of organomercury\u2010contaminated sites.<\/jats:p>","DOI":"10.1111\/j.1467-7652.2006.00236.x","type":"journal-article","created":{"date-parts":[[2007,1,25]],"date-time":"2007-01-25T16:08:53Z","timestamp":1169741333000},"page":"254-262","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":89,"title":["Coupling two mercury resistance genes in Eastern cottonwood enhances the processing of organomercury"],"prefix":"10.1111","volume":"5","author":[{"given":"Satu","family":"Lyyra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard B.","family":"Meagher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tehryung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Heaton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Montello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca S.","family":"Balish","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"Merkle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,1,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00370a064"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002990100317"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.12.6808"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/72678"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.010124"},{"key":"e_1_2_6_7_1","first-page":"530","volume-title":"Silvics of North America","author":"Burns R.M.","year":"1990"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1467-7652.2003.00031.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1079\/IVP2006771"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10653-005-9019-8"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.21273\/HORTSCI.19.4.507"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.2307\/3434144"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00014672"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(03)00155-9"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002990050019"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1897\/02-442"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11270-005-7111-4"},{"key":"e_1_2_6_18_1","first-page":"32p","article-title":"The water\u2010culture method for growing plants without soil","volume":"347","author":"Hoagland D.R.","year":"1950","journal-title":"California Agric. Exp. Stat. Circ"},{"key":"e_1_2_6_19_1","first-page":"41","article-title":"Uprooting toxic waste, cheaply","volume":"264","author":"Ivry B.","year":"2004","journal-title":"Popular Sci"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.065326"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0831166100"},{"key":"e_1_2_6_23_1","volume-title":"SAS for Linear Models","author":"Littell R.C.","year":"2002"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03262926"},{"key":"e_1_2_6_25_1","first-page":"201","volume-title":"Phytoremediation of Contaminated Soil and Water","author":"Meagher R.B.","year":"2000"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.8.3182"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1098-925"},{"key":"e_1_2_6_28_1","first-page":"151","volume-title":"Phytoremediation of Toxic Metals: Using Plants to Clean Up the Environment","author":"Rugh C.L.","year":"2000"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.103.020958"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005854212592"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-003-0682-x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/15226510008500030"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1467-7652.2006.00236.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1467-7652.2006.00236.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T15:57:09Z","timestamp":1695916629000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1467-7652.2006.00236.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1,25]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["10.1111\/j.1467-7652.2006.00236.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1467-7652.2006.00236.x","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1,25]]},"assertion":[{"value":"2007-01-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}