{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T03:06:21Z","timestamp":1789441581852,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,9,13]],"date-time":"2007-09-13T00:00:00Z","timestamp":1189641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We demonstrate that both over\u2010expression and suppression of the gene encoding the morphinan pathway enzyme salutaridinol 7\u2010<jats:italic>O<\/jats:italic>\u2010acetyltransferase (SalAT) in opium poppy affects the alkaloid products that accumulate. Over\u2010expression of the gene in most of the transgenic events resulted in an increase in capsule morphine, codeine and thebaine on a dry\u2010weight basis. The transgenic line with the highest alkaloid content had 41%, 37% and 42% greater total alkaloids than the control in three independent trials over 3 years. DNA\u2010encoded hairpin RNA\u2010mediated suppression of <jats:italic>SalAT<\/jats:italic> resulted in the novel accumulation of the alkaloid salutaridine at up to 23% of total alkaloid; this alkaloid is not detectable in the parental genotype. Salutaridine is not the substrate of SalAT but the substrate of the previous enzyme in the pathway, salutaridine reductase. RNA transcript analysis of 16 primary T<jats:sub>0<\/jats:sub> transformants and their segregating T<jats:sub>1<\/jats:sub> progeny revealed an average reduction in <jats:italic>SalAT<\/jats:italic> transcript to about 12% of the control. Reduction in <jats:italic>SalAT<\/jats:italic> transcript was evident in both leaves and latex. Reverse transcriptase PCR and high\u2010performance liquid chromatography analyses confirmed cosegregation of the expressed transgene with the salutaridine accumulating phenotype.<\/jats:p>","DOI":"10.1111\/j.1467-7652.2007.00293.x","type":"journal-article","created":{"date-parts":[[2007,9,13]],"date-time":"2007-09-13T08:14:08Z","timestamp":1189671248000},"page":"22-30","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":77,"title":["Metabolic engineering of morphinan alkaloids by over\u2010expression and RNAi suppression of salutaridinol 7\u2010<i>O<\/i>\u2010acetyltransferase in opium poppy"],"prefix":"10.1111","volume":"6","author":[{"given":"Robert S.","family":"Allen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A. 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