{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T19:42:23Z","timestamp":1777750943225,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,4,23]],"date-time":"2012-04-23T00:00:00Z","timestamp":1335139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Application of tools of molecular biology and genomics is increasingly leading towards the development of recombinant protein\u2010based biologics. As such, it is leading to an increased diversity of targets that have important health applications and require more flexible approaches for expression because of complex post\u2010translational modifications. For example, <jats:italic>Plasmodium<\/jats:italic> parasites may have complex post\u2010translationally modified proteins such as Pfs48\/45 that do not carry <jats:italic>N<\/jats:italic>\u2010linked glycans (<jats:italic>Exp. Parasitol<\/jats:italic>. 1998; <jats:bold>90<\/jats:bold>, 165.) but contain potential <jats:italic>N<\/jats:italic>\u2010linked glycosylation sites that can be aberrantly glycosylated during expression in mammalian and plant systems. Therefore, it is important to develop strategies for producing non\u2010glycosylated forms of these targets to preserve biological activity and native conformation. In this study, we are describing <jats:italic>in vivo<\/jats:italic> deglycosylation of recombinant <jats:italic>N<\/jats:italic>\u2010glycosylated proteins as a result of their transient co\u2010expression with bacterial PNGase F (Peptide: <jats:italic>N<\/jats:italic>\u2010glycosidase F). In addition, we show that the recognition of an <jats:italic>in vivo<\/jats:italic> deglycosylated plant\u2010produced malaria vaccine candidate, Pfs48F1, by monoclonal antibodies I, III and V raised against various epitopes (I, III and V) of native Pfs48\/45 of <jats:italic>Plasmodium falciparum<\/jats:italic>, was significantly stronger compared to that of the glycosylated form of plant\u2010produced Pfs48F1. To our knowledge, neither <jats:italic>in vivo<\/jats:italic> enzymatic protein deglycosylation has been previously achieved in any eukaryotic system, including plants, nor has bacterial PNGase F been expressed in the plant system. Thus, here, we report for the first time the expression in plants of an active bacterial enzyme PNGase F and the production of recombinant proteins of interest in a non\u2010glycosylated form.<\/jats:p>","DOI":"10.1111\/j.1467-7652.2012.00694.x","type":"journal-article","created":{"date-parts":[[2012,4,23]],"date-time":"2012-04-23T06:52:45Z","timestamp":1335163965000},"page":"773-782","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Production of non\u2010glycosylated recombinant proteins in <i>Nicotiana benthamiana<\/i> plants by co\u2010expressing bacterial PNGase F"],"prefix":"10.1111","volume":"10","author":[{"given":"Tarlan","family":"Mamedov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ananya","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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