{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T17:57:58Z","timestamp":1775152678127,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,1,23]],"date-time":"2008-01-23T00:00:00Z","timestamp":1201046400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["HIV Medicine"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>In heavily pretreated patients, resistance mutations arise in both protease (PR) and reverse transcriptase (RT) sequences; however, the relative impact of PR and RT mutations on viral fitness cannot be evaluated with the majority of systems. To address this issue we have developed a model based on recombinant viruses (RVs) that allows the analysis of the replication capacity (RC) of viral populations in which PR and RT are cloned either in combination or separately.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>RVs were generated for full\u2010length polymerase (<jats:italic>pol<\/jats:italic>) gene, PR or RT sequences from nine na\u00efve and 14 heavily pretreated HIV\u2010infected patients in therapeutic failure. The relative RC was assessed by comparing luciferase activity between mutant RV and wild\u2010type (wt) isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A strong decrease (&gt;60%) in the RC of the <jats:italic>pol<\/jats:italic> RV population was observed in the 14 heavily pretreated patients as compared with the wt RVs. The analysis of PR and RT RVs from these patients showed that the decrease in RC was mainly attributable to PR sequences in three of these 14 patients and to RT sequences in seven of these patients. In the four remaining patients, PR and RT sequences independently reduced the RC of the RVs to similar extents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Different patterns of mutations in either PR or RT have a strong impact on RC in highly experienced HIV\u2010infected patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1468-1293.2007.00540.x","type":"journal-article","created":{"date-parts":[[2008,2,18]],"date-time":"2008-02-18T02:14:56Z","timestamp":1203300896000},"page":"160-171","source":"Crossref","is-referenced-by-count":10,"title":["A new strategy based on recombinant viruses for assessing the replication capacity of HIV\u20101"],"prefix":"10.1111","volume":"9","author":[{"given":"J","family":"Garcia\u2010Perez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Perez\u2010Olmeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Sanchez\u2010Palomino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Perez\u2010Romero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Alcami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,23]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"22","article-title":"Decline in the AIDS and death rates in the EuroSIDA study","volume":"362","author":"Mocroft A","year":"2003","journal-title":"an observational study"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/425075"},{"key":"e_1_2_6_4_2","first-page":"251","article-title":"Treatment for adult HIV infection","volume":"292","author":"Yeni PG","year":"2004","journal-title":"2004 recommendations of the International AIDS Society-USA Panel"},{"key":"e_1_2_6_5_2","first-page":"863","article-title":"Clinical progression and virological failure on highly active antiretroviral therapy in HIV\u20101 patients","volume":"353","author":"Ledergerber B","year":"1999","journal-title":"a prospective cohort study. 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