{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T06:35:00Z","timestamp":1718951700426},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,3,23]],"date-time":"2008-03-23T00:00:00Z","timestamp":1206230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["HIV Medicine"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare flow cytometry assays, using traditional dual platform (DP) or newer single platform (SP) for CD4 enumeration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Records of subjects enrolled in four separate clinical trials using the same central laboratory [SP methodology (Trucount)] were reviewed retrospectively. Eighteen subjects had 60 matching contemporaneous samples at multiple timepoints.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>DP flow cytometry yielded higher CD4 counts in 50\/60 assays (83%). CD4 count and percentage by the two methods showed strong correlation for the counts (<jats:italic>r<\/jats:italic>=0.965, <jats:italic>P<\/jats:italic>&lt;0.0001) and percentages (<jats:italic>r<\/jats:italic>=0.959, <jats:italic>P<\/jats:italic>&lt;0.0001). Bland\u2013Altman plot analysis showed that the limits of variation were within agreeable limits of \u00b12SD in 56\/60 (93.3%) samples tested. Twenty\u2010five (42%) samples had a difference of &gt;50 cells\/\u03bcL. Of these six (24%) exceeded 100 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study is in agreement with previous reports of strong correlation between DP and SP flow cytometry. This review found differences in CD4 counts in a high proportion of samples tested highlighting the importance for clinicians to be aware of such differences when interpreting results from the two methods.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1468-1293.2008.00544.x","type":"journal-article","created":{"date-parts":[[2008,3,24]],"date-time":"2008-03-24T04:01:36Z","timestamp":1206331296000},"page":"192-195","source":"Crossref","is-referenced-by-count":3,"title":["Comparison of different methodologies for CD4 estimation in a clinical setting"],"prefix":"10.1111","volume":"9","author":[{"given":"S","family":"Jeganathan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Bansal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DE","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Gold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,3,23]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199001183220305"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200005113421907"},{"key":"e_1_2_7_4_2","first-page":"1","article-title":"Guidelines for the use of antiretroviral agents in HIV\u2010infected adults and adolescents","volume":"47","author":"Centres for Disease Control.","year":"1998","journal-title":"Morbid Mortal Wkly Rep"},{"key":"e_1_2_7_5_2","first-page":"1","article-title":"Revised classification system for HIV infection and expanded surveillance case definition for AIDS among adolescents and adults","volume":"41","author":"Centres for Disease Control.","year":"1992","journal-title":"Morbid Mortal Wkly Rep"},{"key":"e_1_2_7_6_2","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1128\/CDLI.7.3.333-335.2000","article-title":"Adoption of single\u2010platform technologies for enumeration of absolute T\u2010lymphocyte subsets in peripheral blood","volume":"7","author":"O'Gorman MR","year":"2000","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_7_7_2","first-page":"522, 529","article-title":"Pneumocystis pneumonia in a patient with a high T\u2010cell count","volume":"16","author":"Smith DM.","year":"2006","journal-title":"AIDS Read"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)90837-8"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/1742-6405-3-26"},{"key":"e_1_2_7_10_2","first-page":"1","article-title":"Revised guidelines for performing single\u2010platform absolute CD4 T\u2010cell determinations with CD45 gating for persons infected with human immunodeficiency virus","volume":"52","author":"Mandy F","year":"2003","journal-title":"Morbid Mortal Wkly Rep"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-111-3-223"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.10068"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19991215)38:6<274::AID-CYTO3>3.0.CO;2-I"},{"key":"e_1_2_7_14_2","first-page":"193","article-title":"Comparative assessment of five alternative methods for CD4 enumeration for implementation in developing countries","volume":"26","author":"Didier JM","year":"2001","journal-title":"J AIDS"}],"container-title":["HIV Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1468-1293.2008.00544.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1468-1293.2008.00544.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T18:48:33Z","timestamp":1696877313000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1468-1293.2008.00544.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,3,23]]},"references-count":13,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,4]]}},"alternative-id":["10.1111\/j.1468-1293.2008.00544.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1468-1293.2008.00544.x","archive":["Portico"],"relation":{},"ISSN":["1464-2662","1468-1293"],"issn-type":[{"value":"1464-2662","type":"print"},{"value":"1468-1293","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,3,23]]}}}