{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T17:41:02Z","timestamp":1775151662159,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,11,3]],"date-time":"2011-11-03T00:00:00Z","timestamp":1320278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["HIV Medicine"],"published-print":{"date-parts":[[2012,4]]},"abstract":"Objective<\/jats:title>Drug resistance\u2010associated mutations (DRMs<\/jats:styled-content>) among HIV<\/jats:styled-content>\u20101 treatment\u2010na\u00efve patients have increased in recent years. Their incidence and prevalence in various exposure risk categories (ERCs<\/jats:styled-content>) were evaluated.<\/jats:p><\/jats:sec>Design<\/jats:title>Plasma samples of HIV<\/jats:styled-content>\u20101 treatment\u2010na\u00efve patients diagnosed between 2001 and 2009 at the T<\/jats:styled-content>el A<\/jats:styled-content>viv M<\/jats:styled-content>edical C<\/jats:styled-content>enter were screened for DRMs<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>Samples obtained from patients following the HIV<\/jats:styled-content> diagnosis were analysed retrospectively. Genotyping was carried out using the T<\/jats:styled-content>rugene HIV<\/jats:styled-content>\u20101 genotype kit (S<\/jats:styled-content>iemens, B<\/jats:styled-content>erkeley, CA<\/jats:styled-content>, USA<\/jats:styled-content>). Phylogenetic relationships among viral sequences were estimated using the maximum likelihood method.<\/jats:p><\/jats:sec>Results<\/jats:title>Thirty\u2010eight of the 266 analysed sequences (14.3%) had DRMs<\/jats:styled-content>, all occurring exclusively in the group of men who have sex with men (MSM<\/jats:styled-content>). The rate of DRMs<\/jats:styled-content> has constantly risen, reaching a peak of 21.9% in 2009. Notably, protease inhibitor (PI<\/jats:styled-content>) DRMs<\/jats:styled-content> became the most frequent DRMs<\/jats:styled-content> in 2009. Phylogenetic analysis showed a tight cluster comprising 13 of 14 viruses harbouring the L90M<\/jats:styled-content> major PI<\/jats:styled-content> resistance mutation, suggesting a single infection source.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>There was an unexpectedly high rate of the major L90M<\/jats:styled-content> PI<\/jats:styled-content> resistance mutation in the MSM<\/jats:styled-content> group. The clustered transmission of this mutation might be related to a high\u2010risk sexual behaviour. Added to nonnucleoside reverse transcriptase inhibitor and nucleoside reverse transcriptase inhibitor resistance mutations, such a PI<\/jats:styled-content> mutation may limit future therapeutic options for this particular patient population.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1468-1293.2011.00960.x","type":"journal-article","created":{"date-parts":[[2011,11,4]],"date-time":"2011-11-04T01:32:37Z","timestamp":1320370357000},"page":"202-206","source":"Crossref","is-referenced-by-count":10,"title":["Emergence of an HIV<\/scp>\u20101 cluster harbouring the major protease L90M<\/scp> mutation among treatment\u2010na\u00efve patients in T<\/scp>el A<\/scp>viv, I<\/scp>srael"],"prefix":"10.1111","volume":"13","author":[{"given":"D","family":"Turner","sequence":"first","affiliation":[{"name":"Crusaid Kobler AIDS Center Tel Aviv Sourasky Medical Center Tel Aviv Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Amit","sequence":"additional","affiliation":[{"name":"Crusaid Kobler AIDS Center Tel Aviv Sourasky Medical Center Tel Aviv Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Chalom","sequence":"additional","affiliation":[{"name":"Crusaid Kobler AIDS Center Tel Aviv Sourasky Medical Center Tel Aviv Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O","family":"Penn","sequence":"additional","affiliation":[{"name":"Department of Cell Research and Immunology George S. 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