{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T13:21:41Z","timestamp":1767705701255},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,2,3]],"date-time":"2012-02-03T00:00:00Z","timestamp":1328227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intern J of Cosmetic Sci"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Synopsis<\/jats:title><jats:p>Screening for tyrosinase (TYR) inhibitors potentially useful for control of skin pigmentation has been hampered by the limited availability of human TYR. To overcome this hurdle, we have established human embryonic kidney (HEK293)\u2010TYR cells that constitutively express human TYR. In the current study, we assayed human TYR inhibition activities of 50 plant extracts using the lysates of transformed HEK293\u2010TYR cells. The strongest inhibition of human TYR was shown by the extract of <jats:italic>Vaccinium bracteatum<\/jats:italic> Thunberg, followed by the extract of <jats:italic>Morus bombycis<\/jats:italic> Koidzumi. The former extract did not inhibit mushroom TYR activity whereas significant inhibition was observed with the latter extract, demonstrating the importance of using human TYR in the screening for human TYR inhibitors. Upon liquid\u2010liquid partitioning of the extract from <jats:italic>V.\u00a0bracteatum<\/jats:italic>, the active constituents were enriched in the ethyl acetate fraction, and the subsequent preparatory thin\u2010layer chromatography identified p\u2010coumaric acid (PCA) as the main active constituent. The hypo\u2010pigmentation of PCA was verified in the MelanoDerm\u2122 Skin Model. This study demonstrates that transformed HEK293\u2010TYR cells could expedite the discovery of human TYR\u2010specific inhibitors from natural sources which might be useful in the control of skin pigmentation.<\/jats:p>","DOI":"10.1111\/j.1468-2494.2012.00704.x","type":"journal-article","created":{"date-parts":[[2012,1,6]],"date-time":"2012-01-06T07:20:41Z","timestamp":1325834441000},"page":"202-208","source":"Crossref","is-referenced-by-count":36,"title":["Screening of plant extracts for human tyrosinase inhibiting effects"],"prefix":"10.1111","volume":"34","author":[{"given":"M.","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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