{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:56:38Z","timestamp":1761512198546},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,1,4]],"date-time":"2006-01-04T00:00:00Z","timestamp":1136332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> The analysis of sister chromatid exchange (SCE) is a cytogenetic technique used to show DNA damage as a result of an exchange of DNA fragments between sister chromatids. It is known that there is an increased SCE frequency in Beh\u00e7et's disease (BD).<\/jats:p><jats:p><jats:bold>Objective\u2002<\/jats:bold> To investigate whether human leucocyte antigen (HLA)\u2010B51\u2010positive patients with Beh\u00e7et's disease exhibit higher SCE frequencies than those without HLA\u2010B51.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Lymphocytes from 75 patients (38 women, 37 men) and from 50 controls (28 women, 22 men) were cultured in darkness for 72\u00a0h in the presence of bromodeoxyuridine. Metaphase chromosomes were stained with a fluorescence plus Giemsa technique after a standard harvest procedure. For HLA\u2010B51 typing, DNA was extracted from ethylenediaminetetraacetic acid blood samples and HLA\u2010B5 allele genotyping was performed by the polymerase chain reaction (PCR)\u2013sequence specific primer method.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Thirty\u2010nine of 75 patients with BD (52%) and 15 of 50 controls (30%) were found HLA\u2010B51\u2010positive. The SCE frequencies in HLA\u2010B51\u2010positive patients were higher than in HLA\u2010B51\u2010negative ones (<jats:italic>P\u00a0&lt;<\/jats:italic>\u00a00.001), whereas no difference was detected in the control group.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> This study revealed that there was a significant association between elevated SCE frequencies and existence of HLA\u2010B51 patients with BD.<\/jats:p>","DOI":"10.1111\/j.1468-3083.2006.01386.x","type":"journal-article","created":{"date-parts":[[2006,1,27]],"date-time":"2006-01-27T12:39:04Z","timestamp":1138365544000},"page":"149-152","source":"Crossref","is-referenced-by-count":6,"title":["The alteration of sister chromatid exchange frequencies in Beh\u00e7et's disease with and without 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