{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T13:56:01Z","timestamp":1779890161273,"version":"3.53.1"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,2,9]],"date-time":"2006-02-09T00:00:00Z","timestamp":1139443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> Azathioprine, in combination with corticosteroids, is the first\u2010line therapy of severe forms of pemphigus vulgaris. Patients with an impaired thiopurine <jats:italic>S<\/jats:italic>\u2010methyltransferase (TPMT) activity are at risk of developing severe myelo\u2010suppression upon treatment with thiopurines such as azathioprine. Analysis of the TPMT status prior to drug administration is therefore highly recommended. However, because of the limited availability of TPMT testing outside of specialized centres, pre\u2010emptive TPMT testing is not widespread. To avoid laborious biochemical and sequencing assays, we evaluated a new restriction fragment length polymorphism (RFLP) analysis.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> We designed a rapid genetic polymerase chain reaction (PCR)\u2010RFLP screen for the most prevalent mutant TPMT*3A and TPMT*3C alleles that are known to result in reduced TPMT enzyme activity.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Validating our fast system on 871 Caucasian DNA samples, we observed that 8.61% of our probands carried the TPMT*3A allele and 0.23% were heterozygous for the TPMT*3C allele, which is in concordance with previously reported allele frequencies.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> This simple and low\u2010cost PCR\u2010RFLP TPMT polymorphism testing approach can be performed in a standard laboratory. It should be applied to all patients prior to receiving thiopurine drug therapy to avoid the severe, but predictable, haematopoietic side\u2010effects of thiopurine drug administration.<\/jats:p>","DOI":"10.1111\/j.1468-3083.2006.01459.x","type":"journal-article","created":{"date-parts":[[2006,2,9]],"date-time":"2006-02-09T17:07:59Z","timestamp":1139504879000},"page":"396-400","source":"Crossref","is-referenced-by-count":14,"title":["Introducing a fast and simple PCR\u2010RFLP analysis for the detection of mutant thiopurine <i>S<\/i>\u2010methyltransferase alleles TPMT*3A and 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