{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T21:01:46Z","timestamp":1770152506109,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,6,28]],"date-time":"2006-06-28T00:00:00Z","timestamp":1151452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> The human skin, especially the sebaceous gland, is a steroidogenic organ similar to the gonads and adrenal cortex, possessing all the enzymes required for steroid sex\u2010hormone synthesis and metabolism. Factors regulating cutaneous steroidogenesis associated with disease status remain largely unknown.<\/jats:p><jats:p><jats:bold>Objective\u2002<\/jats:bold> We hypothesized that transcription factors involved in sex formation and regulation of steroidogenesis in the classical steroidogenic organs are also expressed in the sebaceous glands. Their possible role in the pathogenesis of acne were investigated.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> We used reverse transcription polymerase chain reaction (RT\u2010PCR), <jats:italic>in\u00a0situ<\/jats:italic> hybridization and Western blotting to analyse the expression of <jats:italic>SF\u20101<\/jats:italic>, <jats:italic>WT\u20101<\/jats:italic>, <jats:italic>SRY<\/jats:italic>, <jats:italic>SOX\u20109<\/jats:italic> and <jats:italic>DAX\u20101<\/jats:italic> mRNAs and their proteins in cultured human sebocytes and the facial skin of acne patients.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> The <jats:italic>in\u00a0situ<\/jats:italic> hybridization study showed <jats:italic>SOX\u20109<\/jats:italic> mRNA mainly localized in basal keratinocytes, the basal layer of the sebaceous glands and eccrine glands. Immortalized human sebaceous gland cells (SZ95) expressed mRNA for <jats:italic>SOX\u20109<\/jats:italic>, <jats:italic>WT\u20101<\/jats:italic> and <jats:italic>DAX\u20101<\/jats:italic> but not for <jats:italic>SF\u20101<\/jats:italic> or <jats:italic>SRY<\/jats:italic>. The expression of <jats:italic>DAX\u20101<\/jats:italic> protein was slightly inhibited by 10<jats:sup>\u22126<\/jats:sup>\u00a0<jats:sc>m<\/jats:sc> oestradiol (E2) at 6\u00a0h but enhanced by 10<jats:sup>\u22126<\/jats:sup>\u00a0<jats:sc>m<\/jats:sc> dihydrotestosterone (DHT) at 48\u00a0h. The facial expression of <jats:italic>SOX\u20109<\/jats:italic> seemed to be higher in the acne\u2010prone male patients, while <jats:italic>DAX\u20101<\/jats:italic> was stronger in subjects without acne, although both were statistically insignificant.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> Our findings confirm the expression of some sex\u2010determining genes in human sebaceous glands. Further studies on a larger patient population including the normal controls are needed to elucidate the functional significance of these transcription factors in the pathogenesis of acne.<\/jats:p>","DOI":"10.1111\/j.1468-3083.2006.01663.x","type":"journal-article","created":{"date-parts":[[2006,6,28]],"date-time":"2006-06-28T16:27:27Z","timestamp":1151512047000},"page":"846-852","source":"Crossref","is-referenced-by-count":15,"title":["Expression of sex\u2010determining genes in human sebaceous glands and their possible role in the pathogenesis of 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