{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T13:33:55Z","timestamp":1784295235607,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,5,4]],"date-time":"2011-05-04T00:00:00Z","timestamp":1304467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> The pathogenesis of vitiligo remains unclear. Most authorities favoured the autoimmune cause for the strong associations of vitiligo with multiple autoimmune diseases and the presence of autoantibodies in vitiligo patients. Narrow\u2010band UVB (NBUVB) irradiation has been considered to be an effective treatment for vitiligo with simple treatment procedure and decreased accumulated ultraviolet exposure doses.<\/jats:p><jats:p><jats:bold>Objectives\u2002<\/jats:bold> The aim this study was to investigate the effects of NBUVB irradiation on normal IgG antibodies (N\u2010IgG) or vitiligo IgG antibodies (V\u2010IgG)\u2010treated NCCmelan5 cells in terms of proliferation, migration and melanin formation.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Cultured NCCmelan5 cells were treated with (i) NBUVB irradiation alone, (ii) N\u2010IgG or V\u2010IgG alone, and (iii) combination of N\u2010IgG or V\u2010IgG with NBUVB irradiation. The proliferation of NCCmelan5 cells were evaluated using BrdU incorporation assay. Western blotting was used to determine the expressions of phosphorylated p125<jats:sup>FAK<\/jats:sup> (pp125<jats:sup>FAK<\/jats:sup>) and tyrosinase in NCCmelan5 cells. The locomotion of NCCmelan5 cells was assessed using time\u2010lapse assay and <jats:italic>in vitro<\/jats:italic> wound scratch assay.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Neither N\u2010IgG nor V\u2010IgG significantly affected the proliferation of NCCmelan5 cells. The migration, melanin formation and tyrosinase expression in NCCmelan5 cells were decreased by V\u2010IgG. NBUVB irradiation increased the proliferation of V\u2010IgG treated NCCmelan5 cells. In addition, NBUVB irradiation enhanced the mobility of V\u2010IgG\u2010treated NCCmelan5 cells via upregulation of pp125<jats:sup>FAK<\/jats:sup>. The melanogenesis and tyrosinase expression in V\u2010IgG\u2010treated NCCmelan5 cells were promoted using NBUVB irradiation.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> Our study demonstrated that the deleterious effects of V\u2010IgG in the pathogenesis of vitiligo might be overcome by NBUVB irradiation.<\/jats:p>","DOI":"10.1111\/j.1468-3083.2011.04094.x","type":"journal-article","created":{"date-parts":[[2011,5,4]],"date-time":"2011-05-04T08:24:58Z","timestamp":1304497498000},"page":"456-464","source":"Crossref","is-referenced-by-count":19,"title":["Narrow\u2010band UVB irradiation stimulates the migration and functional development of vitiligo\u2010IgG antibodies\u2010treated pigment cells"],"prefix":"10.1111","volume":"26","author":[{"given":"C.\u2010S.","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.\u2010C.E.","family":"Lan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.\u2010S.","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,5,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1977.01640010049006"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(98)70194-X"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-8343(79)90081-1"},{"key":"e_1_2_6_5_2","first-page":"493","article-title":"Response to cosmetic disfigurement: patients with vitiligo","volume":"39","author":"Porter J","year":"1987","journal-title":"Cutis"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780318"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2003.00032.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.2005.00242.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12522891"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(86)70260-0"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12492607"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12461505"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0738-081X(97)00132-6"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.134.12.1532"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-04511-4_7"},{"key":"e_1_2_6_16_2","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1001\/archderm.1997.03890480113020","article-title":"Mechanisms of photochemotherapy of vitiligo","volume":"133","author":"Fitzpatrick TB","year":"1997","journal-title":"Arch Dermatol"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.135.12.1514"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2004.01.007"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2004.02329.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(00)90133-6"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2001.140407.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2007.07.012"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00130.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12324456"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.1984.tb00361.x"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197709222971204"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1600-0749.2003.00119.x"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12289743"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12500086"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.3109\/03009748909095402"},{"key":"e_1_2_6_31_2","first-page":"4827","article-title":"Evaluation of a soluble tetrazolium\/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines","volume":"48","author":"Scudiero DA","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12612795"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12345083"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jidsp.5640194"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.2310\/7750.2008.07054"},{"key":"e_1_2_6_36_2","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1111\/j.1468-3083.2006.02035.x","article-title":"Comparison of systemic PUVA and NB\u2010UVB in the treatment of vitiligo: an open prospective study","volume":"21","author":"Bhatnagar A","year":"2007","journal-title":"J Eur Acad Dermatol Venereol"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2006.01413.x"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2001.114752"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0906-6705.2004.00221.x"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2007.00682.x"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(97)90008-1"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950190208"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2003.12.002"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90385-P"},{"key":"e_1_2_6_45_2","doi-asserted-by":"crossref","first-page":"14545","DOI":"10.1016\/S0021-9258(18)82365-1","article-title":"Cell spreading on extracellular matrix proteins induces tyrosine phosphorylation of tensin","volume":"268","author":"Bockholt SM","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12313396"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041330111"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.1994.tb00635.x"}],"container-title":["Journal of the European Academy of Dermatology and Venereology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1468-3083.2011.04094.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1468-3083.2011.04094.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T09:34:23Z","timestamp":1697362463000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1468-3083.2011.04094.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,5,4]]},"references-count":47,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1111\/j.1468-3083.2011.04094.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1468-3083.2011.04094.x","archive":["Portico"],"relation":{},"ISSN":["0926-9959","1468-3083"],"issn-type":[{"value":"0926-9959","type":"print"},{"value":"1468-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,5,4]]}}}