{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:46:51Z","timestamp":1759333611295},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,10,8]],"date-time":"2011-10-08T00:00:00Z","timestamp":1318032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> Pemphigus vulgaris (PV) is a relatively common autoimmune blistering disease in India. Recently, enzyme\u2010linked immunosorbent assay (ELISA) test against desmoglein1 (Dsg 1) and desmoglein3 (Dsg 3) has been developed, which is found to be highly sensitive and specific.<\/jats:p><jats:p><jats:bold>Objective\u2002<\/jats:bold> To assess the utility of ELISA for monitoring the disease of PV.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Serum samples from 63 active PV patients were tested for anti\u2010Dsg1 and anti\u2010Dsg3 using ELISA. They were tested for anti\u2010Dsg1 and anti\u2010Dsg3 reactivity before treatment. The test was performed during remission period and after relapse using commercially available ELISA kit.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Both anti\u2010Dsg1 and anti\u2010Dsg3 levels were found to be raised in PV patients. Thirty\u2010eight patients went on remission during treatment phase with mean anti\u2010Dsg1 and anti\u2010Dsg3 values, 13.18\u2003U\/mL and 18.89\u2003U\/mL respectively. Difference of baseline from remission was found to be significant (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001). Of these, 23 patients relapsed either during treatment phase or after stoppage of therapy and their mean anti\u2010Dsg1 and anti\u2010Dsg3 titres were 39.49\u2003U\/mL and 96.99\u2003U\/mL respectively. Difference of remission from relapse was also found to be significant (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001). The 50\u2003U\/mL cut\u2010off for anti\u2010Dsg1 ELISA provided 73.91% sensitivity and 72.5% specificity respectively. The 98.57\u2003U\/mL cut\u2010off for anti\u2010Dsg3 provided 82.61% sensitivity and 82.5% specificity respectively.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> ELISA is less time consuming, less expensive and a potential diagnostic test for PV. It is also highly sensitive and specific test for Indian patients in whom the mean anti\u2010Dsg titres are raised.<\/jats:p>","DOI":"10.1111\/j.1468-3083.2011.04294.x","type":"journal-article","created":{"date-parts":[[2011,10,8]],"date-time":"2011-10-08T06:00:31Z","timestamp":1318053631000},"page":"1377-1383","source":"Crossref","is-referenced-by-count":20,"title":["Utility of desmoglein ELISA in the clinical correlation and disease monitoring of pemphigus 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