{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T20:52:07Z","timestamp":1761252727287},"reference-count":17,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2011,11,16]],"date-time":"2011-11-16T00:00:00Z","timestamp":1321401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> Former studies have shown that <jats:italic>Propionibacterium acnes<\/jats:italic> may stimulate expression of toll\u2010like receptor 4 (TLR4) in keratinocytes of patients with acne vulgaris.<\/jats:p><jats:p><jats:bold>Objective\u2002<\/jats:bold> To investigate the impact of single nucleotide polumorphisms (SNPs) of the <jats:italic>TLR4<\/jats:italic> gene in acne vulgaris.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Genomic DNA was isolated from 191 patients with acne vulgaris and 75 healthy controls. Asp299Gly and Thr399Ile SNPs were defined after cutting of the PCR products by restriction enzymes. Sebum of lesions was cultured for <jats:italic>P. acnes<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> No differences in SNP allele frequencies were found between patients and healthy controls. 46.5% of carriers of wild\u2010type alleles were suffering from acne conglobata compared with 28.6% of carriers of SNP alleles (<jats:italic>P<\/jats:italic>\u2003=\u20030.040). After adjusting for gender, family history of acnes, intake of any therapy and skin isolation of <jats:italic>P. acnes<\/jats:italic>, carriage of <jats:italic>TLR4<\/jats:italic> gene SNPs was the only independent variable linked with a protective role against acne conglobata (OR\u2003=\u20030.269, <jats:italic>P<\/jats:italic>\u2003=\u20030.014). No differences were found in the amount of pro\u2010inflammatory cytokines released by peripheral blood mononuclear cells isolated from patients with acne conglobata carrying only wild\u2010type alleles and SNP alleles.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> Carriage of gene SNPs is protective against the development of acne conglobata even in the presence of <jats:italic>P. acnes<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1468-3083.2011.04338.x","type":"journal-article","created":{"date-parts":[[2011,11,16]],"date-time":"2011-11-16T20:48:24Z","timestamp":1321476504000},"page":"1538-1543","source":"Crossref","is-referenced-by-count":11,"title":["Single nucleotide polymorphisms of toll\u2010like receptor\u20104 protect against acne conglobata"],"prefix":"10.1111","volume":"26","author":[{"given":"I.","family":"Grech","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Giatrakou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Damoraki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Pistiki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Kaldrimidis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.J.","family":"Giamarellos\u2010Bourboulis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Stavrianeas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,11,16]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2010.03919.x"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.c5812"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2005.06527.x"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sder.2010.04.002"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000067818"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.015"},{"key":"e_1_2_5_8_2","first-page":"497","article-title":"Expression, subcellular localization and cytokinic modulation of Toll\u2010like receptors (TLRs) in normal human keratinocytes: TLR2 up\u2010regulation in psoriatic skin","volume":"17","author":"Begon E","year":"2007","journal-title":"Eur J Dermatol"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016594"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2005.06933.x"},{"key":"e_1_2_5_11_2","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1093\/genetics\/158.4.1657","article-title":"Excess of rare amino acid polymorphisms in the Toll\u2010like receptor 4 in humans","volume":"158","author":"Smirnova I","year":"2001","journal-title":"Genetics"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2004.03.012"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1701-2163(16)32915-2"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704828104"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2004.03.008"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1159\/000095057"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.281"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2007.06.045"}],"container-title":["Journal of the European Academy of Dermatology and Venereology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1468-3083.2011.04338.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1468-3083.2011.04338.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T08:37:20Z","timestamp":1697359040000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1468-3083.2011.04338.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11,16]]},"references-count":17,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/j.1468-3083.2011.04338.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1468-3083.2011.04338.x","archive":["Portico"],"relation":{},"ISSN":["0926-9959","1468-3083"],"issn-type":[{"value":"0926-9959","type":"print"},{"value":"1468-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11,16]]}}}