{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T22:20:53Z","timestamp":1783549253748,"version":"3.55.0"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,3,13]],"date-time":"2012-03-13T00:00:00Z","timestamp":1331596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glucocorticoids have been implicated in male reproductive function and 11\u03b2\u2010HSD\u20101 and \u20102, the glucocorticoid receptor (GR) and mineralocorticoid receptor (MR), all of which are known to modulate glucocorticoid action, have been localised in the adult rat epididymis, but their developmental expression has not been investigated. Na<jats:sup>+<\/jats:sup>K<jats:sup>+<\/jats:sup>\u2010ATPase activity, responsible for sodium transport, is induced by both mineralocorticoids and glucocorticoids in the kidney and colon, and has been localised in epididymal epithelium. This study examined the immunolocalisation of 11\u03b2\u2010HSD\u20101 and \u20102, GR, MR and Na<jats:sup>+<\/jats:sup>K<jats:sup>+<\/jats:sup>\u2010ATPase in rat epididymal epithelium (<jats:italic>n\u2003<\/jats:italic>=\u20035) at postnatal days (pnd) 1, 7, 15, 28, 40, 60, 75 and 104, and relative mRNA expression of 11\u03b2\u2010HSD\u20101 and \u20102, and GR at pre\u2010puberty (pnd 28) and post\u2010puberty (pnd 75). 11\u03b2\u2010HSD\u20101, GR and MR were localised in the epididymal epithelium from pnd 1, and 11\u03b2\u2010HSD\u20102 and Na<jats:sup>+<\/jats:sup>K<jats:sup>+<\/jats:sup>\u2010ATPase reactivity from pnd 15. At pnd 28 there was maximal immunoreactivity for both the GR and MR and 11\u03b2\u2010HSD\u20101 and \u20102. 11\u03b2\u2010HSD\u20101 mRNA expression in the caput increased from pre\u2010 to post\u2010puberty, whereas 11\u03b2\u2010HSD\u20102 mRNA expression fell over the same period (<jats:italic>P\u2003<\/jats:italic>&lt;\u20030.01). GR mRNA expression was similar at pre\u2010 and post\u2010puberty in both caput and cauda. Developmental changes in expression of 11\u03b2\u2010HSD\u20101 and \u20102 suggest that overall exposure of the epididymis to glucocorticoids increases post\u2010puberty, but cell\u2010specific expression of the 11\u03b2\u2010HSD enzymes still provides a capacity for intricate local control of glucocorticoid exposure.<\/jats:p>","DOI":"10.1111\/j.1469-7580.2012.01481.x","type":"journal-article","created":{"date-parts":[[2012,3,13]],"date-time":"2012-03-13T17:03:50Z","timestamp":1331658230000},"page":"350-362","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Immunolocalisation of 11\u03b2\u2010HSD\u20101 and \u20102, glucocorticoid receptor, mineralocorticoid receptor and Na<sup>+<\/sup>K<sup>+<\/sup>\u2010ATPase during the postnatal development of the rat epididymis"],"prefix":"10.1111","volume":"220","author":[{"given":"Gwyneth H.","family":"Gladstones","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Burton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Mark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan J.","family":"Waddell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Roberts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,13]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gap059"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1983.tb00119.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1987.tb00952.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"7799","DOI":"10.1016\/S0021-9258(19)38999-9","article-title":"Enhanced intracellular sodium concentration in the kidney cells recruits a latent pool of Na\u2010K\u2010ATPase whose size is modulated by corticosteroids","volume":"265","author":"Barlet\u2010Bas C","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/JBCPP.2007.18.2.115"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.142.4.1644"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1071\/BI9830205"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.137.12.5700"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001880105"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1992.03480090092034"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0910293"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10312"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2000.279.6.F1132"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.20332"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006909927287"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-127-1-32"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(85)90242-0"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(08)60337-3"},{"key":"e_1_2_5_20_1","first-page":"28","article-title":"Is the epididymis a kidney analogue?","volume":"3","author":"Hinton BT","year":"1988","journal-title":"News Physiol Sci"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20383"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092340206"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000050588.35034.3C"},{"key":"e_1_2_5_24_1","first-page":"73","article-title":"Plasma membrane structure and remodelling during sperm maturation in the epididymis","volume":"53","author":"Jones RC","year":"1998","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_5_25_1","first-page":"388","article-title":"Corticosteroid regulation of NaK\u2010ATPase along the mammalian nephron","volume":"10","author":"Katz AI","year":"1990","journal-title":"Semin Nephrol"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.130.5.3074"},{"key":"e_1_2_5_27_1","first-page":"330","article-title":"Action of glucocorticoids on proximal tubule transport systems","volume":"10","author":"Kinsella JL","year":"1990","journal-title":"Semin Nephrol"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(98)00256-1"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0890-6238(02)00002-3"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1987.233"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2005.12.033"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0083-6729(08)60447-1"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-5-1-25"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9313"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756360412331295026"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-118-5-2072"},{"key":"e_1_2_5_37_1","first-page":"999","volume-title":"The Physiology of Reproduction","author":"Robaire B","year":"1988"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.121.2.589"},{"key":"e_1_2_5_39_1","doi-asserted-by":"crossref","first-page":"17\u2003643","DOI":"10.1016\/S0021-9258(19)77885-5","article-title":"Thyroid hormone regulates \u03b1 and \u03b1+ isoforms of Na,K\u2010ATPase during development in neonatal rat brain","volume":"263","author":"Schmitt CA","year":"1988","journal-title":"J Biol Chem"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(93)90036-J"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(01)00633-5"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-07-0126"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2009.03.009"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.05.013"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.138.2.540"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001540104"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10311"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1995.tb00532.x"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1994.tb00476.x"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2003.10.069"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-0082"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00806"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0150093"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.135.3.901"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00005899"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002323200"}],"container-title":["Journal of Anatomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1469-7580.2012.01481.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1469-7580.2012.01481.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T08:26:47Z","timestamp":1698654407000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1469-7580.2012.01481.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,13]]},"references-count":55,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1111\/j.1469-7580.2012.01481.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1469-7580.2012.01481.x","archive":["Portico"],"relation":{},"ISSN":["0021-8782","1469-7580"],"issn-type":[{"value":"0021-8782","type":"print"},{"value":"1469-7580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,13]]},"assertion":[{"value":"2012-03-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}