{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T22:40:17Z","timestamp":1759963217335},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,3,14]],"date-time":"2012-03-14T00:00:00Z","timestamp":1331683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Salivary secretion is principally regulated by autonomic nerves. However, recent evidence from <jats:italic>in vivo<\/jats:italic> animal experiments suggests that gastrointestinal peptide hormones can also influence saliva production. The aim of the present study was to define the secretagogue activity of the gastrin\u2010analogue pentagastrin in human salivary glands. For this purpose, parotid tissues were exposed to pentagastrin <jats:italic>in vitro<\/jats:italic>. Morphological techniques were used to evaluate modifications to serous acinar cells associated with secretion. Using a variant of the osmium maceration method, high resolution scanning electron microscopy allowed assessment of the morphology of the cytoplasmic aspect of the plasmalemma to demonstrate secretory activity. To quantify responses to pentagastrin, we recorded morphometric data on microvilli, microbuds, and protrusions. Dose\u2010dependent morphological changes were observed, whereas protein concentration increased in the incubate. The use of selective receptor antagonists showed pentagastrin to act principally <jats:italic>via<\/jats:italic> cholecystokinin\u2010A receptors. The morphological responses observed following exposure to pentagastrin differed from those elicited following exposure to the pan\u2010muscarinic agonist carbachol. This study provides the first demonstration of a direct secretory action of gastrointestinal peptides on salivary glands in humans.<\/jats:p>","DOI":"10.1111\/j.1469-7580.2012.01489.x","type":"journal-article","created":{"date-parts":[[2012,3,14]],"date-time":"2012-03-14T05:26:34Z","timestamp":1331702794000},"page":"447-453","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Morphological evidence that pentagastrin regulates secretion in the human parotid gland"],"prefix":"10.1111","volume":"220","author":[{"given":"Francesco","family":"Loy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Diana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raffaella","family":"Isola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Solinas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"Isola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriele","family":"Conti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Serenella","family":"Lantini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margherita","family":"Cossu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Riva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rgen","family":"Ekstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020107915919"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1998.tb09639.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.1242\/jcs.109.10.2591","article-title":"Two regulated secretory pathways for newly synthesized parotid salivary proteins are distinguished by doses of secretagogues","volume":"109","author":"Castle JD","year":"1996","journal-title":"J Cell Sci"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090010301"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"2963","DOI":"10.1242\/jcs.115.14.2963","article-title":"The minor regulated pathway, a rapid component of salivary secretion, may provide docking\/fusion sites for granule exocytosis at the apical surface of acinar cells","volume":"115","author":"Castle AM","year":"2002","journal-title":"J Cell Sci"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2006.01.004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2006.033639"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2006.034710"},{"key":"e_1_2_7_10_1","first-page":"95","article-title":"Melatonin\u2010induced protein synthesis in the rat parotid gland","volume":"62","author":"\u00c7evik\u2010Aras H","year":"2011","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_7_11_1","first-page":"323","article-title":"Autonomic control of salivary secretion","volume":"85","author":"Ekstr\u00f6m J","year":"1989","journal-title":"Proc Finn Dent Soc"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","unstructured":"Ekstr\u00f6mJ(1999)Role of nonadrenergic noncholinergic autonomic transmitters in salivary glandular activitiesin vivo. 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