{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:43:16Z","timestamp":1792654996987,"version":"4.5.0"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,9,30]],"date-time":"2004-09-30T00:00:00Z","timestamp":1096502400000},"content-version":"vor","delay-in-days":2556,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1997,10]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The whole\u2010cell configuration of the patch\u2010clamp technique was used to study the effects of immunoglobulins (IgGs) from patients affected by amyotrophic lateral sclerosis (ALS) on spontaneous glutamatergic currents in rat hippocampal cells in culture.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Focal application of ALS IgGs (100 \u03bcg ml<jats:sup>\u22121<\/jats:sup>) to hippocampal cells induced a rise in frequency but not in amplitude of spontaneous excitatory postsynaptic currents (SEPSC) which outlasted the period of IgG application. The mean frequency ratio (ALS over control) was 3.2 \u00b1 0.6 (<jats:italic>n.<\/jats:italic> 19). No changes in frequency or amplitude of SEPSCs were observed after treatment with IgGs obtained from healthy donors (<jats:italic>n<\/jats:italic>=5) or from patients with Alzheimer's disease (<jats:italic>n<\/jats:italic>=4).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>ALS IgGs also increased the frequency (by a factor of 2.0 \u00b1 0.3) but not the amplitude of miniature excitatory postsynaptic currents (mEPSC) recorded in the presence of TTX (<jats:italic>n<\/jats:italic>=19). A rise in frequency of mEPSC was also seen in cells superfused with a calcium\u2010free solution (<jats:italic>n<\/jats:italic>=4).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the presence of TTX, ALS IgGs did not modify the amplitude or the shape of currents evoked by AMPA (100 \u03bc<jats:sc>m<\/jats:sc>), recorded at a holding potential of \u221250 mV.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>It is concluded that ALS IgGs enhance both SEPSCs and mEPSCs through a presynaptic type of action. The excessive release of glutamate from nerve endings may be the cause of motoneurone death in ALS patients.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/j.1469-7793.1997.103bf.x","type":"journal-article","created":{"date-parts":[[2004,9,30]],"date-time":"2004-09-30T03:45:11Z","timestamp":1096515911000},"page":"103-112","source":"Crossref","is-referenced-by-count":56,"title":["Immunoglobulins from motoneurone disease patients enhance glutamate release from rat hippocampal neurones in culture"],"prefix":"10.1113","volume":"504","author":[{"given":"Pavle R.","family":"Andjus","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zorica","family":"Stevic\u2010Marinkovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Cherubim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,9,30]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199608120-00008"},{"key":"e_1_2_5_2_1","first-page":"2142","article-title":"Immunoglobulins from patients affected by amyotrophic lateral sclerosis increase the frequency of spontaneous glutamatergic currents in cultured rat hippocampal neurones","volume":"22","author":"Andjus P. 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