{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:56:16Z","timestamp":1773420976124,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,9,30]],"date-time":"2004-09-30T00:00:00Z","timestamp":1096502400000},"content-version":"vor","delay-in-days":2556,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1997,10]]},"abstract":"<jats:p><jats:list list-type=\"explicit-label\"><jats:list-item><jats:p>In newborn mammals, systemic hypoxia provokes catecholamine secretion from the adrenal medulla. In contrast to adults, this release is independent of sympathetic innervation. We have studied the cellular processes involved in hypoxia\u2010induced catecholamine secretion, employing fluorimetric techniques to measure changes in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>, NADH and mitochondrial potential, and voltammetric techniques to record changes in<jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub>and catecholamine secretion.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>In adrenal chromaffin cells freshly dissociated from newborn rats, severe hypoxia increased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>and secretion of catecholamines, indicating that the response of the newborn adrenal medulla to hypoxia is an intrinsic property of these cells. Discrete quantal secretory events were identifiable, suggesting an exocytotic mechanism of secretion.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Hypoxia\u2010induced secretion was only seen when<jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub>fell below 5 mmHg, similar to the threshold arterial<jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub>reported to stimulate release<jats:italic>in vivo.<\/jats:italic>Such oxygen tensions also inhibited mitochondrial metabolism, shown by an increase in NADH autofluorescence. We therefore explored the involvement of mitochondria in oxygen sensing. Inhibition of mitochondrial respiration either by CN<jats:sup>\u2212<\/jats:sup>at complex IV or by rotenone at complex I mimicked severe hypoxia, reversibly increasing both [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>and catecholamine secretion. The CN<jats:sup>\u2212<\/jats:sup>\u2010induced depolarization of the mitochondrial inner membrane potential preceded the increase in [Ca<jats:sup>2<\/jats:sup>]<jats:sub>i<\/jats:sub>by \u223c6 s.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>The effects of severe hypoxia and CN<jats:sup>\u2212<\/jats:sup>on [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>and catecholamine secretion were not additive, suggesting a common mechanism.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Chemical anoxia failed to increase [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in a significant proportion of cells dissociated from 2\u2010 to 4\u2010week\u2010old rats. Thus, the sensitivity to hypoxia is specific to adrenal chromaffin cells dissociated from newborn rats.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>These data indicate that hypoxia\u2010induced catecholamine secretion in the newborn adrenal medulla is mediated by reversible inhibition of mitochondrial respiration, leading to an increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>and catecholamine secretion.<\/jats:p><\/jats:list-item><\/jats:list><\/jats:p>","DOI":"10.1111\/j.1469-7793.1997.175bf.x","type":"journal-article","created":{"date-parts":[[2004,9,30]],"date-time":"2004-09-30T07:45:11Z","timestamp":1096530311000},"page":"175-189","source":"Crossref","is-referenced-by-count":114,"title":["Hypoxia\u2010induced catecholamine secretion in isolated newborn rat adrenal chromaffin cells is mimicked by inhibition of mitochondrial respiration"],"prefix":"10.1113","volume":"504","author":[{"given":"Mart H.","family":"Mojet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elliott","family":"Mills","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael R.","family":"Duchen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,9,30]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5687(94)90043-4"},{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.73.6.1100"},{"key":"e_1_2_5_3_1","first-page":"213","volume-title":"Noninvasive Techniques in Cell Biology","author":"Balaban R. 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