{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T22:57:42Z","timestamp":1770332262209,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,9,30]],"date-time":"2004-09-30T00:00:00Z","timestamp":1096502400000},"content-version":"vor","delay-in-days":2495,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1997,12]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>We have investigated the origin of the intracellular acid pH transients that accompany myometrial contraction. Intra\u2010and extracellular pH were measured with SNARF and intra\u2010cellular Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) with indo\u20101.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>An intracellular acidification accompanied spontaneous contractions and those elicited by KC1 depolarization or the addition of the agonists carbachol or prostaglandin F<jats:sub>2x<\/jats:sub>. The size of the acidification increased with the magnitude of the contraction.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The intracellular acidification was accompanied by an extracellular <jats:italic>alkalinization<\/jats:italic>, showing that it results from proton movement across the surface membrane. Furthermore, it was decreased either by addition of Cd<jats:sup>2+<\/jats:sup> (20 n<jats:sc>m<\/jats:sc>, an inhibitor of the sarcolemmal Ca<jats:sup>2+<\/jats:sup>\u2010ATPase) or by elevating [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>o<\/jats:sub>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Extracellular alkalinization increased the magnitude of the rise of [Ca<jats:sup>2+<\/jats:sup>]j and force produced by KC1.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>An intracellular acidification was also associated with contraction in the portal vein and ureter.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We conclude that the sarcolemmal Ca<jats:sup>2+<\/jats:sup>\u2010ATPase produces a significant intracellular acidification while removing Ca<jats:sup>2+<\/jats:sup>. Both the acidification and decrease of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> will promote relaxation. Since Ca<jats:sup>2+<\/jats:sup> and protons have opposite effects on many cellular processes, this dual regulation by these two ions may be of general importance.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/j.1469-7793.1997.329bb.x","type":"journal-article","created":{"date-parts":[[2004,9,30]],"date-time":"2004-09-30T07:17:30Z","timestamp":1096528650000},"page":"329-336","source":"Crossref","is-referenced-by-count":22,"title":["The role of the sarcolemmal Ca2<sup>+<\/sup> \u2010ATPase in the pH transients associated with contraction in rat smooth muscle"],"prefix":"10.1113","volume":"505","author":[{"given":"E. K.","family":"Naderali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Buttell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"Taggart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"Bullock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. A.","family":"Eisner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Wray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,9,30]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1991.261.6.C1010"},{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016145"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020966"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021429"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019697"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117819"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1991.sp003548"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2520215"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7292(93)90368-7"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00003a031"},{"key":"e_1_2_5_11_1","first-page":"148","volume-title":"The Uterus","author":"Kawarabayashi T.","year":"1994"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod40.5.942"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.07.030184.001353"},{"key":"e_1_2_5_14_1","first-page":"56P","article-title":"The effects of contraction on intracellular pH in the rat uterus","volume":"495","author":"Naderali E. 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