{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:32:03Z","timestamp":1773300723944,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item>\n<jats:p>The effects of cytosolic ATP on sarcoplasmic reticulum (SR) Ca<jats:sup>2+<\/jats:sup> regulation were investigated in saponin\u2010permeabilised rat ventricular myocytes. [Ca<jats:sup>2+<\/jats:sup>] within the cells was monitored using Fura\u20102 or Fluo\u20103 fluorescence. Spontaneous cyclic Ca<jats:sup>2+<\/jats:sup> release from the SR was induced by increasing the bathing [Ca<jats:sup>2+<\/jats:sup>] to 200\u2013300 nM, in solutions weakly Ca<jats:sup>2+<\/jats:sup> buffered with 0.05 m<jats:sc>m<\/jats:sc> EGTA. Alternatively, Ca<jats:sup>2+<\/jats:sup>\u2010induced Ca<jats:sup>2+<\/jats:sup> release (CICR) was triggered by a rapid increase in [Ca<jats:sup>2+<\/jats:sup>] induced by flash photolysis of Nitr\u20105 (0.08 m<jats:sc>m<\/jats:sc>), replacing EGTA in the solution.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Stepwise reductions in [ATP] were associated with corresponding decreases in the frequency and increases in the amplitude of spontaneous Ca<jats:sup>2+<\/jats:sup> transients. A decrease from 5 m<jats:sc>m<\/jats:sc> to 0.1 m<jats:sc>m<\/jats:sc> ATP, reduced the release frequency by 48.6 \u00b1 7 % (<jats:styled-content><jats:italic>n<\/jats:italic>= 7<\/jats:styled-content>) and almost doubled the amplitude of the Ca<jats:sup>2+<\/jats:sup> transient. Marked prolongation of the spontaneous Ca<jats:sup>2+<\/jats:sup> transient occurred when [ATP] was further reduced to 10 \u03bcM, consistent with inhibition of the SR Ca<jats:sup>2+<\/jats:sup> pump.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>These effects of ATP were compared with other interventions that inhibit Ca<jats:sup>2+<\/jats:sup> uptake or reduce the sensitivity of the SR Ca<jats:sup>2+<\/jats:sup> release mechanism. Inhibition of the SR Ca<jats:sup>2+<\/jats:sup> pump with cyclopiazonic acid (CPA) markedly reduced the spontaneous Ca<jats:sup>2+<\/jats:sup> release frequency, without changing the amplitude. The descending phase of the Ca<jats:sup>2+<\/jats:sup> transient was prolonged in the presence of CPA, while the rising phase was unaffected. In contrast, desensitisation of the SR Ca<jats:sup>2+<\/jats:sup> release mechanism with tetracaine decreased the frequency of spontaneous release, but markedly increased the amplitude.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>CICR triggered by flash photolysis of Nitr\u20105 appeared to be more sensitive to cytosolic [ATP] than spontaneous release and was generally delayed by a decrease to 2.5 m<jats:sc>m<\/jats:sc> ATP. In the presence of 0.1\u20100.2 m<jats:sc>m<\/jats:sc> ATP, release often failed completely or was not consistently triggered. Some preparations exhibited Ca<jats:sup>2+<\/jats:sup> release \u2018alternans\u2019, whereby every alternate trigger induced a response.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>These results suggest that the increase in spontaneous Ca<jats:sup>2+<\/jats:sup> release amplitude and the decrease in frequency that occurs as [ATP] is reduced from 1 m<jats:sc>m<\/jats:sc> to 100 \u03bcM, is mainly due to desensitisation of the SR Ca<jats:sup>2+<\/jats:sup> release mechanism, which allows the SR Ca<jats:sup>2+<\/jats:sup> content to reach a higher level before release occurs. At very low [ATP], a reduction in the SR Ca<jats:sup>2+<\/jats:sup> uptake rate may also contribute to the decrease in release frequency. CICR triggered by photolysis of Nitr\u20105 appeared to be more sensitive to cytosolic [ATP]. The possible underlying mechanisms and the relevance of these results to myocardial ischaemia or hypoxia is considered.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/j.1469-7793.2000.00029.x","type":"journal-article","created":{"date-parts":[[2004,8,13]],"date-time":"2004-08-13T07:40:14Z","timestamp":1092382814000},"page":"29-44","source":"Crossref","is-referenced-by-count":30,"title":["Effects of cytosolic ATP on spontaneous and triggered Ca<sup>2+<\/sup>\u2010induced Ca<sup>2+<\/sup> release in permeabilised rat ventricular myocytes"],"prefix":"10.1113","volume":"523","author":[{"given":"Zhaokang","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek S.","family":"Steele","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,2]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.60.2.153"},{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"C1313","DOI":"10.1152\/ajpcell.1995.268.5.C1313","article-title":"Fractional SR Ca2+ release is regulated by trigger Ca2+ and SR Ca2+ content in cardiac myocytes","volume":"37","author":"Bassani J. 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