{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:47:02Z","timestamp":1761058022053},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,8,5]],"date-time":"2004-08-05T00:00:00Z","timestamp":1091664000000},"content-version":"vor","delay-in-days":1131,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2001,7]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The expression of ClC\u20103 was examined in rat lacrimal gland and submandibular salivary gland cells using RT\u2010PCR and Western analysis. Whole\u2010cell patch clamp methods were used to investigate the expression of volume\u2010sensitive anion channels in acinar cells isolated from these tissues.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Expression of mRNA encoding ClC\u20103, and ClC\u20103 protein, was found in rat submandibular gland by RT\u2010PCR and Western analysis. Rat lacrimal gland cells, however, did not appear to express mRNA encoding for ClC\u20103, nor the ClC\u20103 protein.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Volume\u2010sensitive anion conductances were observed in both rat lacrimal gland and submandibular salivary gland acinar cells. The conductance was of a similar size in the two cell types, but was much slower to activate in the lacrimal cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The properties of the conductances in lacrimal and submandibular cells were similar, e.g. halide selectivity sequence (<jats:italic>P<\/jats:italic><jats:sub>I<\/jats:sub> &gt; <jats:italic>P<\/jats:italic><jats:sub>Cl<\/jats:sub> &gt; <jats:italic>P<\/jats:italic><jats:sub>aspartate<\/jats:sub>) and inhibition by 4,4\u2032\u2010diisothiocyanostilbene\u20102,2\u2032\u2010disulphonic acid, 5\u2010nitro\u20102\u2010(3\u2010phenylpropylamino)\u2010benzoate and tamoxifen.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The data suggest that the expression of ClC\u20103 is not an absolute requirement for the activity of volume\u2010sensitive anion channels in rat lacrimal gland acinar cells.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/j.1469-7793.2001.00409.x","type":"journal-article","created":{"date-parts":[[2004,8,5]],"date-time":"2004-08-05T09:53:45Z","timestamp":1091699625000},"page":"409-421","source":"Crossref","is-referenced-by-count":17,"title":["Expression of volume\u2010sensitive Cl<sup>\u2212<\/sup> channels and ClC\u20103 in acinar cells isolated from the rat lacrimal gland and submandibular salivary gland"],"prefix":"10.1113","volume":"534","author":[{"given":"Aneela","family":"Majid","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter 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