{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T07:16:36Z","timestamp":1769325396174,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,8,5]],"date-time":"2004-08-05T00:00:00Z","timestamp":1091664000000},"content-version":"vor","delay-in-days":1131,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2001,7]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Serotonin (5\u2010HT) facilitates the connections between sensory and motor neurons in <jats:italic>Aplysia<\/jats:italic> during behavioural sensitization. The effect of 5\u2010HT on sensorimotor synapses is believed to be primarily presynaptic. Here we tested whether 5\u2010HT can have an exclusively postsynaptic facilitatory effect.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Siphon motor neurons were individually dissociated from the abdominal ganglion of <jats:italic>Aplysia<\/jats:italic> and placed into cell culture. Brief pulses of glutamate, the putative sensory neuron transmitter, were focally applied (0.1 Hz) to solitary motor neurons in culture, and the glutamate\u2010evoked postsynaptic potentials (Glu\u2010PSPs) were recorded.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>When 5\u2010HT was perfused over the motor neuron for 10 min, the amplitude of the Glu\u2010PSPs was significantly increased. The 5\u2010HT\u2010induced enhancement of the Glu\u2010PSPs persisted for at least 40 min after washout.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Prior injection into the motor neuron of the calcium chelator BAPTA, GDP\u2010\u03b2\u2010S or GTP\u2010\u03b3\u2010S blocked the 5\u2010HT\u2010induced facilitation of the Glu\u2010PSPs. However, the facilitation was not blocked when APV, an NMDA receptor antagonist, was applied together with the 5\u2010HT.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The enhancement of the Glu\u2010PSPs by 5\u2010HT was reversed by the AMPA receptor antagonist DNQX, indicating that 5\u2010HT increased the functional expression of AMPA\u2010type receptors in the motor neuron.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The presence of botulinum toxin in the motor neuron blocked the 5\u2010HT\u2010induced enhancement of the Glu\u2010PSPs. As botulinum toxin prevents exocytosis we hypothesize that during sensitization 5\u2010HT causes the insertion of additional AMPA\u2010type receptors into the postsynaptic membrane of sensorimotor synapses via exocytosis. This postsynaptic mechanism may contribute to facilitation of the synapses.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/j.1469-7793.2001.00501.x","type":"journal-article","created":{"date-parts":[[2004,8,5]],"date-time":"2004-08-05T09:53:45Z","timestamp":1091699625000},"page":"501-510","source":"Crossref","is-referenced-by-count":71,"title":["Serotonin facilitates AMPA\u2010type responses in isolated siphon motor neurons of <i>Aplysia<\/i> in culture"],"prefix":"10.1113","volume":"534","author":[{"given":"Raymond A.","family":"Chitwood","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L.","family":"Glanzman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,8,5]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"crossref","first-page":"8770","DOI":"10.1523\/JNEUROSCI.18-21-08770.1998","article-title":"Presynaptic induction and expression of homosynaptic depression at Aplysia sensorimotor neuron synapses","volume":"18","author":"Armitage B. A.","year":"1998","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-01-00458.1998"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/361315a0"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-02-00425.1996"},{"key":"e_1_2_5_5_1","first-page":"1526","article-title":"Serotonin enhances the glutamate response in isolated Aplysia motor neurons in culture","volume":"26","author":"Chitwood R. A.","year":"2000","journal-title":"Society for Neuroscience Abstracts"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(199905)39:2<237::AID-NEU8>3.0.CO;2-0"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.15.7163"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.480190402"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.09-12-04200.1989"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90046-2"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/31496"},{"key":"e_1_2_5_12_1","first-page":"1525","article-title":"Serotonin\u2010induced enhancement of glutamate response in Aplysia motor neurons: dependence upon G protein activation and exocytosis of postsynaptic receptors","volume":"26","author":"Li Q.","year":"2000","journal-title":"Society for Neuroscience Abstracts"},{"key":"e_1_2_5_13_1","article-title":"The role of postsynaptic calcium and postsynaptic exocytosis in serotonin\u2010induced facilitation of Aplysia sensorimotor synapses","volume":"27","author":"Li Q.","year":"2001","journal-title":"Society for Neuroscience Abstracts"},{"key":"e_1_2_5_14_1","doi-asserted-by":"crossref","first-page":"7585","DOI":"10.1523\/JNEUROSCI.15-11-07585.1995","article-title":"Cloning and characterization of two related serotonergic receptors from the brain and the reproductive system of Aplysia that activate phospholipase C","volume":"15","author":"LI X. C.","year":"1995","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/375400a0"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1994.0016"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1994.0031"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5349.399"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.09-12-04227.1989"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(00)00099-4"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.18.9931"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5337.467"},{"key":"e_1_2_5_23_1","article-title":"Role of postsynaptic calcium and postsynaptic exocytosis in sensitization of the siphon\u2010withdrawal reflex in Aplysia","volume":"27","author":"Roberts A. C.","year":"2001","journal-title":"Society for Neuroscience Abstracts"},{"key":"e_1_2_5_24_1","first-page":"597","volume":"17","author":"Schacher S.","year":"1997","journal-title":"Pathway-specific synaptic plasticity: activity-dependent enhancement and suppression of long-term heterosynaptic facilitation at converging inputs on a single target"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5421.1811"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/375645a0"},{"key":"e_1_2_5_27_1","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1152\/jn.1993.70.3.1221","article-title":"Excitatory amino acid neurotransmission at sensory\u2010motor and interneuronal synapses of Aplysia californica","volume":"70","author":"Trudeau L. E.","year":"1993","journal-title":"Journal of Neurophysiology"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-02-01275.1995"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1063(2000)10:1<57::AID-HIPO6>3.0.CO;2-0"},{"key":"e_1_2_5_30_1","doi-asserted-by":"crossref","first-page":"4976","DOI":"10.1523\/JNEUROSCI.17-13-04976.1997","article-title":"Site\u2010specific and sensory neuron\u2010dependent increases in postsynaptic glutamate sensitivity accompany serotonin\u2010induced long\u2010term facilitation at Aplysia sensorimotor synapses","volume":"17","author":"Zhu H.","year":"1997","journal-title":"Journal of Neuroscience"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1469-7793.2001.00501.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1469-7793.2001.00501.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T04:52:51Z","timestamp":1697259171000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1111\/j.1469-7793.2001.00501.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,7]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,7]]}},"alternative-id":["10.1111\/j.1469-7793.2001.00501.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1469-7793.2001.00501.x","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,7]]}}}