{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:20:21Z","timestamp":1791829221998,"version":"4.3.4"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,12,22]],"date-time":"2003-12-22T00:00:00Z","timestamp":1072051200000},"content-version":"vor","delay-in-days":661,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJOG"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> 1. To assess the contribution of the sarcoplasmic reticulum calcium store in the generation of uterine smooth muscle contractions; 2. to evaluate the contribution of calcium induced calcium release or ryanodine gated calcium channels to myometrial force production.<\/jats:p><jats:p><jats:bold>Design\n\t\t\t\t\t<\/jats:bold> Laboratory scientific study.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Myometrial strips were obtained from women undergoing elective prelabour caesarean section at term. These were loaded with the calcium sensitive indicator Indo\u20101 allowing simultaneous assessment of intracellular calcium concentrations and force production. The effect of exposing the strips to ryanodine (which abolishes calcium induced calcium release), caffeine (which activates calcium induced calcium release) and cyclopiazonic acid (which abolishes the sarcoplasmic *reticulum calcium store) was examined.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> Exposure to ryanodine had no appreciable effect on either the amplitude or the duration of the myometrial calcium and force transients but did increase the frequency of contractions (139 \u00b1 5%). Caffeine did not potentiate force. Cyclopiazonic acid increased frequency, duration and amplitude of both calcium and force transients. The ability of oxytocin to provoke calcium and force transients in the absence of extracellular calcium was abolished by cyclopiazonic acid but not by ryanodine.<\/jats:p><jats:p><jats:bold>Conclusions\n\t\t\t\t\t<\/jats:bold> These results demonstrate that calcium induced calcium release does not play a significant role in human myometrium and that no functioning role for the ryanodine receptors in human myometrial tissue could be shown. These data suggest that the sarcoplasmic reticulum may act to limit contractions and act as a calcium sink, rather than to amplify contractions.<\/jats:p>","DOI":"10.1111\/j.1471-0528.2002.01110.x","type":"journal-article","created":{"date-parts":[[2003,12,22]],"date-time":"2003-12-22T16:25:19Z","timestamp":1072110319000},"page":"289-296","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Effect of inhibiting the sarcoplasmic reticulum on spontaneous and oxytocin\u2010induced contractions of human myometrium"],"prefix":"10.1111","volume":"109","author":[{"given":"S.","family":"Kupittayanant","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.J.M.","family":"Luckas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Wray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,12,22]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.133bi.x"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(99)00104-4"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)00924-X"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.277.2.C243"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(99)70580-6"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.3.748"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.00153.x"},{"key":"e_1_2_7_9_2","first-page":"39P","article-title":"Inhibiting the sarcoplasmic reticulum in human uterus does not decrease contraction","volume":"526","author":"Kupittayanant S","year":"2000","journal-title":"J Physiol"},{"key":"e_1_2_7_10_2","first-page":"90P","article-title":"Reconstitution of single ryanodine\u2010sensitive cation channels from rat myometrial microsomal membranes","volume":"487","author":"Martin C","year":"1995","journal-title":"J Physiol"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1990.tb14691.x"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018092"},{"key":"e_1_2_7_13_2","first-page":"73","article-title":"Mg2+, ATP\u2010dependent Ca2+ transport in endoplasmic reticulum of myometrial cells [Russian]","volume":"61","author":"Kosterin SA","year":"1996","journal-title":"Biokhimiia"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0855s.x"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(86)90639-4"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2000.tb11626.x"},{"key":"e_1_2_7_17_2","first-page":"1077","article-title":"The caffeine\u2010 and ryanodine\u2010sensitive Ca2+ store in porcine myometrial cells: its heterogeneity of all\u2010or\u2010none Ca2+ release","volume":"275","author":"Zhu Ge R","year":"1995","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_7_18_2","first-page":"39","article-title":"Different actions of some relaxant agents acting via the cAMP system in rat uterus","volume":"17","author":"Chulia S","year":"1995","journal-title":"Methods Find Exp Clin Pharmacol"},{"key":"e_1_2_7_19_2","first-page":"53","article-title":"Mg2+, ATP\u2010dependent Ca2+ transport in endoplasmic reticulum of myometrial cells","volume":"61","author":"Kosterin SA","year":"1996","journal-title":"Biochem"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.803bg.x"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-0575-0_7"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13848.x"},{"key":"e_1_2_7_23_2","first-page":"266","article-title":"Capacitative Ca2+ entry and the regulation of smooth muscle tone","volume":"19","author":"Gibson A","year":"1998","journal-title":"Trends Pharmacol Sci"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0707h.x"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1999.276.2.E345"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1994.tb13201.x"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1360497"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(95)00089-4"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4160(96)90049-4"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199811000-00001"}],"container-title":["BJOG: An International Journal of Obstetrics &amp; 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