{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:10:45Z","timestamp":1791231045645,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2003,12,22]],"date-time":"2003-12-22T00:00:00Z","timestamp":1072051200000},"content-version":"vor","delay-in-days":477,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJOG"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To comparatively analyse the expression of matrix metalloproteinase (MMP\u20103) and tissue inhibitor of MMP (TIMP\u20102) in serosal tissue of intraperitoneal organs and adhesions, as well as peritoneal fluid and serum of subjects with and without adhesions.<\/jats:p><jats:p><jats:bold>Design\n\t\t\t\t\t<\/jats:bold> Cross sectional study.<\/jats:p><jats:p><jats:bold>Setting\n\t\t\t\t\t<\/jats:bold> Academic research centres.<\/jats:p><jats:p><jats:bold>Sample\n\t\t\t\t\t<\/jats:bold> Patients undergoing abdominal\/pelvic surgery.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Messenger ribonucleic acid (mRNA) and protein expression using quantitative reverse transcription polymerase chain reaction (Q\u2010RT\u2010PCR), ELISA and immunohistochemistry.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> All the tissues examined express MMP\u20103 and TIMP\u20102 mRNA and protein at consistently varying levels ranging from 100\u2010 to 1000\u2010fold and 2\u2010 to 10\u2010fold mRNA and protein, respectively. Serosa of uterine, fallopian tube, ovary and partial peritoneum express a higher MMP\u20103 mRNA compared with small and large bowels and omentum, while TIMP\u20102 expression was less variable with the highest level found in uterine serosa (<jats:styled-content><jats:italic>P<\/jats:italic> &lt; 0.05<\/jats:styled-content>). The expression of MMP\u20103 and TIMP\u20102 mRNA in adhesions was not significantly different compared with parietal peritoneum. MMP\u20103 and TIMP\u20102 protein content in tissue extracts, peritoneal fluids and serum also varied with higher TIMP\u20102 than MMP\u20103 (<jats:styled-content><jats:italic>P<\/jats:italic> &lt; 0.05<\/jats:styled-content>). TIMP\u20102 levels were lower in serum of subjects with moderate\/extensive adhesions compared with subjects without adhesions. Immunoreactive MMP\u20103 and TIMP\u20102 proteins were detected in various cell types in these tissues. There was no correlation between MMP\u20103 and TIMP\u20102 expression, and age, gender or menstrual status of subjects with or without adhesions.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> MMP\u20103 and TIMP\u20102 are expressed at varying levels in serosal tissues of peritoneal organs and adhesions, with higher TIMP\u20102 than MMP\u20103. Based on the knowledge that tissue injury alters the expression of MMPs and TIMPs, such variations may predispose an organ to develop more adhesions than others. In addition, the results suggest that serum level of TIMP\u20102 may represent a marker for subjects who will form adhesions with greater severity.<\/jats:p>","DOI":"10.1111\/j.1471-0528.2002.01334.x","type":"journal-article","created":{"date-parts":[[2003,12,22]],"date-time":"2003-12-22T15:31:23Z","timestamp":1072107083000},"page":"1041-1049","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Differential expression of matrix metalloproteinase and tissue inhibitor of MMP in serosal tissue of intraperitoneal organs and adhesions"],"prefix":"10.1111","volume":"109","author":[{"given":"Nasser","family":"Chegini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Kotseos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunfeng","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Bennett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael 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