{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T23:29:36Z","timestamp":1789946976868,"version":"4.0.1"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,12,10]],"date-time":"2009-12-10T00:00:00Z","timestamp":1260403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJOG"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p><jats:bold>Objective\u2002<\/jats:bold> Pre\u2010eclampsia involves a maternal inflammatory response that differs from both normal pregnancy and normotensive intrauterine growth restriction (IUGR). Our objective was to examine neutrophil Toll\u2010like receptor (TLR), cryopyrin, nuclear factor\u2010\u03baB (NF\u2010\u03baB) subunit and interleukin\u20101\u03b2 (IL\u20101\u03b2), and inflammatory cytokine profiles in women with pre\u2010eclampsia or normotensive IUGR, as well as in normal pregnancy and non\u2010pregnancy controls.<\/jats:p><jats:p><jats:bold>Design and method\u2002<\/jats:bold> A case\u2013control study was performed. We examined the messenger RNA (mRNA) and protein expressions of TLR4 and TLR2, mRNA levels of cryopyrin, IL\u20101\u03b2, NF\u2010\u03baB subunits p50 and p65, as well as maternal serum inflammatory cytokine profiles (IL\u20102, IL\u20106, tumour necrosis factor\u2010\u03b1 [TNF\u2010\u03b1], interferon\u2010\u03b3 [IFN\u2010\u03b3] and IL\u201010) in women with and without pre\u2010eclampsia using real\u2010time reverse transcription polymerase chain reactions, flow cytometry and multiplex immunoassays.<\/jats:p><jats:p><jats:bold>Setting\u2002<\/jats:bold> A single tertiary maternity hospital in Vancouver, Canada.<\/jats:p><jats:p><jats:bold>Population\u2002<\/jats:bold> Women with early\u2010onset pre\u2010eclampsia (&lt;34\u2003weeks of gestation, <jats:italic>n<\/jats:italic>\u2003=\u200325), women with late\u2010onset pre\u2010eclampsia (\u226534<jats:sup>+0<\/jats:sup>\u2003weeks of gestation, <jats:italic>n<\/jats:italic>\u2003=\u200325), women with normotensive IUGR (<jats:italic>n<\/jats:italic>\u2003=\u200325), women with normal pregnancy (<jats:italic>n<\/jats:italic>\u2003=\u200375) and non\u2010pregnancy (<jats:italic>n<\/jats:italic>\u2003=\u200325) controls.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Women with pre\u2010eclampsia (as a single combined group of early\u2010 and late\u2010onset, and particularly in women with early\u2010onset pre\u2010eclampsia) had increased TLR2 and TLR4 mRNA and protein expressions elevated cryopyrin, NF\u2010\u03baB subunit, and IL\u20101\u03b2 mRNA expression, and TNF\u2010\u03b1:IL\u201010 and IL\u20106:IL\u201010 ratios compared with other groups.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> These data suggest that TLRs and cryopyrin may modulate the innate immune response of the maternal syndrome of pre\u2010eclampsia, and might also trigger the differential inflammatory response existing between early onset pre\u2010eclampsia and normotensive IUGR.<\/jats:p>","DOI":"10.1111\/j.1471-0528.2009.02428.x","type":"journal-article","created":{"date-parts":[[2010,6,25]],"date-time":"2010-06-25T14:48:11Z","timestamp":1277477291000},"page":"99-108","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Toll\u2010like receptors 2 and 4 and the cryopyrin inflammasome in normal pregnancy and 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