{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T04:49:04Z","timestamp":1754110144769},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T00:00:00Z","timestamp":1160006400000},"content-version":"vor","delay-in-days":7613,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[1985,12]]},"abstract":"<jats:p><jats:bold>Abstract: <\/jats:bold> Four hybridoma lines secreting monoclonal antibodies to benzodiazepines were produced after BALB\/c mice were immunized with a benzodiazepine\u2010bovine serum albumin conjugate. The monoclonal antibodies were purified from ascites fluids, and their binding affinities for benzodiazepines and other benzodiazepine receptor ligands were determined. These antibodies have very high binding affinities for diazepam, flunitrazepam, Ro5\u20104864, Ro5\u20103453, Ro11\u20136896, and Ro5\u20133438 (the <jats:italic>K<\/jats:italic><jats:sub>D<\/jats:sub> values are in the 10<jats:sup>\u22129<\/jats:sup><jats:italic>M<\/jats:italic> range). However, these antibodies have low affinities for the benzodiazepine receptor inverse agonists (\u03b2\u2010carbolines) and antagonists (Ro15\u20131788 and CGS\u20108216).<\/jats:p>","DOI":"10.1111\/j.1471-4159.1985.tb10530.x","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T18:48:13Z","timestamp":1160074093000},"page":"1748-1753","source":"Crossref","is-referenced-by-count":28,"title":["Monoclonal Antibodies to Benzodiazepines"],"prefix":"10.1111","volume":"45","author":[{"given":"Angel L.","family":"De Blas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakshmi","family":"Sangameswaran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven A.","family":"Haney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongeun","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Carl J.","family":"Abraham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carroll A.","family":"Rayner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,5]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.9.3805"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/15563658108990290"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.19.040179.002531"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/305056a0"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(81)90205-2"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(84)90118-5"},{"key":"e_1_2_2_8_1","first-page":"11","volume-title":"Quantitative Analytic Studies in Epilepsy","author":"Silva J. 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