{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:44:27Z","timestamp":1792651467255,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,6,4]],"date-time":"2004-06-04T00:00:00Z","timestamp":1086307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nicotinic acetylcholine receptors (nAChRs) mediate fast synaptic transmission in the insect brain and are target sites for neonicotinoid insecticides. Seven nAChR subunits (four \u03b1\u2010type and three \u03b2\u2010type) have been cloned previously from<jats:italic>Drosophila melanogaster<\/jats:italic>, the model insect system and characterized by heterologous expression. Recently, three further putative nAChR \u03b1 subunits (D\u03b15, D\u03b16 and D\u03b17) with sequence similarity to the vertebrate \u03b17 subunit have been identified from<jats:italic>Drosophila<\/jats:italic>genome sequence data but there have been no reports, as yet, of their characterization by heterologous expression. In the present study, we report the first isolation of a full\u2010length D\u03b17 cDNA and the independent molecular cloning of D\u03b16. Binding of nicotinic radioligands was not detected to full\u2010length D\u03b16 or D\u03b17 subunits when expressed alone or when or co\u2010expressed with other nAChR subunits in<jats:italic>Drosophila<\/jats:italic>or mammalian cell lines, but specific cell\u2010surface binding of [<jats:sup>125<\/jats:sup>I]\u03b1\u2010bungarotoxin (<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>\u2003=\u20030.68\u2003\u00b1\u20030.22\u2003n<jats:sc>m<\/jats:sc>) and [<jats:sup>3<\/jats:sup>H]methyllycaconitine (<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>\u2003=\u20030.27\u2003\u00b1\u20030.06\u2003n<jats:sc>m<\/jats:sc>) was detected after expression of a subunit chimera containing the ligand\u2010binding domains of D\u03b16 fused to the C\u2010terminal domain of the 5\u2010hydroxytryptamine receptor 5HT<jats:sub>3A<\/jats:sub>. Although cell\u2010surface binding was not detected with a D\u03b17\/5HT<jats:sub>3\u0391<\/jats:sub>chimera expressed alone, co\u2010expression of the two subunit chimeras resulted in significantly enhanced levels of nicotinic radioligand binding (with no change in affinity). This is the first evidence for the formation of a nAChR binding site by heterologously expressed<jats:italic>Drosophila<\/jats:italic>nAChR subunits in the absence of a co\u2010expressed vertebrate nAChR subunit. In addition to the formation of homomeric nAChR complexes, evidence has been obtained from both radioligand binding and co\u2010immunoprecipitation studies for the co\u2010assembly of D\u03b16 and D\u03b17 into heteromeric cell surface complexes.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2004.02499.x","type":"journal-article","created":{"date-parts":[[2004,7,2]],"date-time":"2004-07-02T06:12:45Z","timestamp":1088748765000},"page":"479-489","source":"Crossref","is-referenced-by-count":64,"title":["Molecular characterization of D\u03b16 and D\u03b17 nicotinic acetylcholine receptor subunits from<i>Drosophila<\/i>: formation of a high\u2010affinity \u03b1\u2010bungarotoxin binding site revealed by expression of subunit chimeras"],"prefix":"10.1111","volume":"90","author":[{"given":"Stuart J.","family":"Lansdell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil S.","family":"Millar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,6,7]]},"reference":[{"key":"e_1_2_25_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1994.tb00997.x"},{"key":"e_1_2_25_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb02854.x"},{"key":"e_1_2_25_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.3.1043"},{"key":"e_1_2_25_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)02057-3"},{"key":"e_1_2_25_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-3042.2001.00685.x"},{"key":"e_1_2_25_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1998.70010349.x"},{"key":"e_1_2_25_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.68052140.x"},{"key":"e_1_2_25_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1998.70062585.x"},{"key":"e_1_2_25_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.38.27145"},{"key":"e_1_2_25_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90344-F"},{"key":"e_1_2_25_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(98)00221-4"},{"key":"e_1_2_25_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(78)90084-3"},{"key":"e_1_2_25_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/366479a0"},{"key":"e_1_2_25_15_1","first-page":"212","article-title":"Homomers of \u03b18 and \u03b17 subunits of nicotinic receptors exhibit similar channels but contrasting binding site properties","volume":"45","author":"Gerzanich V.","year":"1994","journal-title":"Mol. 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