{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:30:00Z","timestamp":1759336200756},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,8,31]],"date-time":"2004-08-31T00:00:00Z","timestamp":1093910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nicastrin was the first binding partner of presenilin (PS) shown to be a critical component of the presenilin\/\u03b3\u2010secretase complex essential in development and differentiation, and in generation of Alzheimer's disease A\u03b2 amyloid peptide. To investigate the function of this glycoprotein, we compared nicastrin and presenilin protein expression in various mouse tissues. Western blot analysis of PS1, PS2 and nicastrin indicates their expression levels are not coordinated. In adult mouse, nicastrin is highly expressed in muscle membranes, whereas presenilin levels are very low. By Blue Native electrophoresis, a PS1 complex of 400\u2003kDa was detected in lung, brain, thymus and heart; nicastrin was also detected as a 400\u2010kDa complex in brain but in muscle it was detected with a complex mobility of 240 and 290\u2003kDa, suggesting association with alternate protein complexes. Immunocytochemistry confirms strong intracellular expression of nicastrin in skeletal muscle and blood vessel smooth muscle. These findings suggest a function for nicastrin in muscle other than participation in the \u03b3\u2010secretase complex.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2004.02718.x","type":"journal-article","created":{"date-parts":[[2006,3,3]],"date-time":"2006-03-03T12:16:14Z","timestamp":1141388174000},"page":"230-237","source":"Crossref","is-referenced-by-count":14,"title":["Nicastrin expression in mouse peripheral tissues is not co\u2010ordinated with presenilin and is high in muscle"],"prefix":"10.1111","volume":"91","author":[{"given":"Nancy T.","family":"Ilaya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Genevi\u00e8ve","family":"Evin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin L.","family":"Masters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janetta G.","family":"Culvenor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,9,6]]},"reference":[{"key":"e_1_2_17_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1101-1030"},{"key":"e_1_2_17_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)02865-6"},{"key":"e_1_2_17_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi034045z"},{"key":"e_1_2_17_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64538-5"},{"key":"e_1_2_17_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(85)90727-1"},{"key":"e_1_2_17_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1999.4791"},{"key":"e_1_2_17_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200207190-00018"},{"key":"e_1_2_17_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03936.x"},{"key":"e_1_2_17_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.132277899"},{"key":"e_1_2_17_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb960"},{"key":"e_1_2_17_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(03)00005-9"},{"key":"e_1_2_17_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(01)01789-3"},{"key":"e_1_2_17_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.6030"},{"key":"e_1_2_17_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm910"},{"key":"e_1_2_17_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi035748j"},{"key":"e_1_2_17_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00189-2"},{"key":"e_1_2_17_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1999.1147"},{"key":"e_1_2_17_19_1","unstructured":"GuY. 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