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In this study, we tested their effects in a model of neonatal hypoxia\u2010ischemia (HI) in 7\u2010day\u2010old rats (unilateral carotid ligation and exposure to 7.7% O<jats:sub>2<\/jats:sub> for 50\u2003min). EPO (10\u2003U\/g body weight\u2003=\u200380\u2003ng\/g; <jats:italic>n<\/jats:italic>\u2003=\u200324), asialoEPO (80\u2003ng\/g; <jats:italic>n<\/jats:italic>\u2003=\u200323) or vehicle (phosphate\u2010buffered saline with 0.1% human serum albumin; <jats:italic>n<\/jats:italic>\u2003=\u200324) was injected intraperitoneally 4\u2003h before HI. Both drugs were protective, as judged by measuring the infarct volumes, neuropathological score and gross morphological score. The infarct volumes were significantly reduced by both EPO (52%) and asialoEPO (55%) treatment, even though the plasma levels of asialoEPO had dropped below the detection limit (1\u2003p<jats:sc>m<\/jats:sc>) at the onset of HI, while those of EPO were in the nanomolar range. Thus, a brief trigger by asialoEPO before the insult appears to be sufficient for protection. Proteomics analysis after asialoEPO treatment alone (no HI) revealed at least one differentially up\u2010regulated protein, synaptosome\u2010associated protein of 25\u2003kDa (SNAP\u201025). Activation (phosphorylation) of ERK was significantly reduced in asialoEPO\u2010treated animals after HI. EPO and the nonerythropoietic asialoEPO both provided significant and equal neuroprotection when administered 4\u2003h prior to HI in 7\u2010day\u2010old rats. The protection might be related to reduced ERK activation and up\u2010regulation of SNAP\u201025.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2004.02769.x","type":"journal-article","created":{"date-parts":[[2004,11,2]],"date-time":"2004-11-02T16:36:27Z","timestamp":1099413387000},"page":"900-910","source":"Crossref","is-referenced-by-count":98,"title":["The nonerythropoietic asialoerythropoietin protects against neonatal hypoxia\u2010ischemia as potently as erythropoietin"],"prefix":"10.1111","volume":"91","author":[{"given":"Xiaoyang","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changlian","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens Gammeltoft","family":"Gerwien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre","family":"Schrattenholz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mats","family":"Sandberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Leist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klas","family":"Blomgren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,27]]},"reference":[{"key":"e_1_2_22_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0387-7604(03)00039-1"},{"key":"e_1_2_22_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1510"},{"key":"e_1_2_22_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199906000-00007"},{"key":"e_1_2_22_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007807200"},{"key":"e_1_2_22_6_1","first-page":"79","article-title":"What evidence supports use of erythropoietin as a novel neurotherapeutic?","volume":"16","author":"Brines M.","year":"2002","journal-title":"Oncology (Huntingt.)"},{"key":"e_1_2_22_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.19.10526"},{"key":"e_1_2_22_8_1","first-page":"97","article-title":"Recombinant human erythropoietin (rHuEPO): more than just the correction of uremic anemia","volume":"15","author":"Buemi M.","year":"2002","journal-title":"J. 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