{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:09:56Z","timestamp":1777468196879,"version":"3.51.4"},"reference-count":54,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,27]],"date-time":"2004-10-27T00:00:00Z","timestamp":1098835200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>NGFI\u2010B (Nur77\/Nr4a1) is a member of a nuclear steroid receptor subgroup that includes the related factors Nurr1 (Nr4a2) and NOR\u20101 (Nr4a3). These proteins do not have recognized ligands and in fact function independently as orphan receptors with transcriptional regulatory activity. In the present study, expression of the <jats:italic>NGFI\u2010B<\/jats:italic> gene in the rat pineal gland was found to exhibit a robust circadian rhythm, with elevated levels of <jats:italic>NGFI\u2010B<\/jats:italic> mRNA occurring at night. The rhythm of <jats:italic>NGFI\u2010B<\/jats:italic> mRNA is translated into a circadian rhythm of NGFI\u2010B protein, which accumulates in the nucleus of pinealocytes. In addition, there is a parallel marked nocturnal increase in pineal DNA binding activity to a NGFI\u2010B response element (NBRE, AAAGGTCA). Pharmacological studies indicate that <jats:italic>NGFI\u2010B<\/jats:italic> mRNA and protein levels are elevated <jats:italic>via<\/jats:italic> activation of adrenergic receptors. NGFI\u2010B protein levels are also elevated by dibutyryl cyclic AMP, as in other systems. In the pineal gland, regulation of <jats:italic>NGFI\u2010B<\/jats:italic> expression also involves the AP\u20101 protein Fra\u20102, based on studies with a transgenic Fra\u20102 knockdown rat, in which pineal NGFI\u2010B expression increases. This set of observations extends the number of pineal genes that are known to be regulated by Fra\u20102, and also provides the first indication that a member of the NGFI\u2010B group of nuclear receptors is involved in controlling gene expression in the pineal gland.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2004.02777.x","type":"journal-article","created":{"date-parts":[[2004,11,3]],"date-time":"2004-11-03T08:56:12Z","timestamp":1099472172000},"page":"946-955","source":"Crossref","is-referenced-by-count":34,"title":["NGFI\u2010B (Nurr77\/Nr4a1) orphan nuclear receptor in rat pinealocytes: circadian expression involves an adrenergic\u2010cyclic AMP mechanism"],"prefix":"10.1111","volume":"91","author":[{"given":"Ann","family":"Humphries","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan","family":"Weller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruben","family":"Baler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Carter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,27]]},"reference":[{"key":"e_1_2_17_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1990.9999"},{"key":"e_1_2_17_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.9.2499"},{"key":"e_1_2_17_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00420-3"},{"key":"e_1_2_17_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.45.27319"},{"key":"e_1_2_17_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0517"},{"key":"e_1_2_17_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.11.6979"},{"key":"e_1_2_17_8_1","first-page":"248","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein\u2010dye binding","volume":"72","author":"Bradford M.","year":"1976","journal-title":"Biochemistry"},{"key":"e_1_2_17_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.133.5.2263"},{"key":"e_1_2_17_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(94)90358-1"},{"key":"e_1_2_17_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5242.1681"},{"key":"e_1_2_17_12_1","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1124\/mol.51.4.551","article-title":"Regulation of pineal alpha1B\u2010adrenergic receptor mRNA: day\/night rhythm and beta\u2010adrenergic receptor\/cyclic AMP control","volume":"51","author":"Coon S. L.","year":"1997","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_17_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.8.4331"},{"key":"e_1_2_17_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.12.6454"},{"key":"e_1_2_17_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M305905200"},{"key":"e_1_2_17_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.141.7.2392"},{"key":"e_1_2_17_17_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-079X.2003.00078.x"},{"key":"e_1_2_17_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.20.5.689"},{"key":"e_1_2_17_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.22.8444"},{"key":"e_1_2_17_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.11.6.3239"},{"key":"e_1_2_17_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.141.12.4496"},{"key":"e_1_2_17_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0007-1331.2001.00738.x"},{"key":"e_1_2_17_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.140.3.1272"},{"key":"e_1_2_17_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/35017072"},{"key":"e_1_2_17_25_1","first-page":"307","article-title":"The melatonin rhythm\u2010generating enzyme: molecular regulation of serotonin N\u2010acetyltransferase in the pineal gland","volume":"52","author":"Klein D.","year":"1997","journal-title":"Recent Prog. Horm. Res."},{"key":"e_1_2_17_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend.16.7.0863"},{"key":"e_1_2_17_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1066"},{"key":"e_1_2_17_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/367281a0"},{"key":"e_1_2_17_29_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-80-12-3073"},{"key":"e_1_2_17_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.11.7549"},{"key":"e_1_2_17_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.3.763-776.2003"},{"key":"e_1_2_17_32_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-09-03326.1999"},{"key":"e_1_2_17_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90138-9"},{"key":"e_1_2_17_34_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend.11.1.9874"},{"key":"e_1_2_17_35_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend-4-10-1438"},{"key":"e_1_2_17_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051003198"},{"key":"e_1_2_17_37_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.7.769"},{"key":"e_1_2_17_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.10.5946"},{"key":"e_1_2_17_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.10.5952"},{"key":"e_1_2_17_40_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.137.11.4691"},{"key":"e_1_2_17_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg620"},{"key":"e_1_2_17_42_1","first-page":"439","article-title":"Norepinephrine stimulation of pineal cyclic AMP response element\u2010binding protein phosphorylation: primary role of a beta\u2010adrenergic receptor\/cyclic AMP mechanism","volume":"47","author":"Roseboom P. H.","year":"1995","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_17_43_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_17_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.11.3704-3713.2001"},{"key":"e_1_2_17_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0932598100"},{"key":"e_1_2_17_46_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.4459"},{"key":"e_1_2_17_47_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5260.419"},{"key":"e_1_2_17_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.10.5427"},{"key":"e_1_2_17_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.9.10.4213"},{"key":"e_1_2_17_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.10.6124"},{"key":"e_1_2_17_51_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1925541"},{"key":"e_1_2_17_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/367277a0"},{"key":"e_1_2_17_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.12.7731"},{"key":"e_1_2_17_54_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5440.790"},{"key":"e_1_2_17_55_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5310.248"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1471-4159.2004.02777.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1471-4159.2004.02777.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:05:15Z","timestamp":1695773115000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1471-4159.2004.02777.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,10,27]]},"references-count":54,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,11]]}},"alternative-id":["10.1111\/j.1471-4159.2004.02777.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1471-4159.2004.02777.x","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,10,27]]}}}