{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:14:31Z","timestamp":1791188071754,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,4,20]],"date-time":"2005-04-20T00:00:00Z","timestamp":1113955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Stromal cell\u2010derived factor\u20101\u03b1 (SDF\u20101\u03b1) is a chemokine whose receptor, CXCR4, is distributed in specific brain areas including hypothalamus. SDF\u20101\u03b1 has recently been found to play important roles in neurons, although direct modulation of voltage\u2010gated ionic channels has never been shown. In order to clarify this issue, we performed patch\u2010clamp experiments in fetal mouse hypothalamic neurons in culture. SDF\u20101\u03b1 (10\u2003n<jats:sc>m<\/jats:sc>) decreased the peak and rising slope of the action potentials and spike discharge frequency in 22% of hypothalamic neurons tested. This effect was blocked by the CXCR4 antagonist AMD\u20033100 (1\u2003\u00b5<jats:sc>m<\/jats:sc>) but not by the metabotropic glutamate receptor antagonist MCPG (500\u2003\u00b5<jats:sc>m<\/jats:sc>), indicating a direct action of SDF\u20101\u03b1 on its cognate receptor. This effect involved a depression of both inward and outward voltage\u2010dependent currents of the action potential. We confirmed these effects in the human neuroblastoma cell line SH\u2010SY5Y, which endogenously expresses CXCR4. Voltage\u2010clamp experiments revealed that SDF\u20101\u03b1 induced a 20% decrease in the peak of the tetrodotoxin\u2010sensitive sodium current and tetraethylammonium\u2010sensitive delayed rectifier potassium current, respectively. Both effects were concentration dependent, and blocked by AMD\u20033100 (200\u2003n<jats:sc>m<\/jats:sc>). This dual effect was reduced or blocked by 0.4\u2003m<jats:sc>m<\/jats:sc> GTP\u03b3S G\u2010protein pre\u2010activation or by pre\u2010treatment with the G\u2010protein inhibitor pertussis toxin (200\u2003ng\/mL), suggesting that it is mediated via activation of a G<jats:sub>i\/o<\/jats:sub>\u2003protein. This study extends the functions of SDF\u20101\u03b1 to a direct modulation of voltage\u2010dependent membrane currents of neuronal cells.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2005.03083.x","type":"journal-article","created":{"date-parts":[[2005,4,27]],"date-time":"2005-04-27T15:22:06Z","timestamp":1114615326000},"page":"963-973","source":"Crossref","is-referenced-by-count":39,"title":["Stromal cell\u2010derived factor\u20101\u03b1 directly modulates voltage\u2010dependent currents of the action potential in mammalian neuronal cells"],"prefix":"10.1111","volume":"93","author":[{"given":"A.","family":"Guyon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Rov\u00e8re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Cervantes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Allaeys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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