{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T16:07:51Z","timestamp":1764173271081},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The amyloid\u2010\u03b2 precursor protein (APP) is linked to Alzheimer's disease through its pathological proteolytic processing in the secretory pathway. Nevertheless, surprisingly little is known about the biosynthesis of endogenous APP. We therefore decided to investigate the intracellular fate of newly synthesized APP in a physiologically inducible neuroendocrine cell, the <jats:italic>Xenopus<\/jats:italic> intermediate pituitary melanotrope cell. We found that the level of both APP mRNA and protein was about threefold induced in the activated cells of black\u2010adapted animals. Intriguingly, two pools of APP were found, only one of which was up\u2010regulated. This induced pool became readily <jats:italic>N<\/jats:italic>\u2010 and subsequently <jats:italic>O<\/jats:italic>\u2010glycosylated and was eventually proteolytically processed by an \u03b1\u2010secretase\u2010like cleavage event resulting in a secreted N\u2010terminal and a cell\u2010associated C\u2010terminal APP fragment. Conversely, only the other (non\u2010induced, non\u2010glycosylated and uncleaved) pool became phosphorylated. Thus, we report on the biosynthesis of APP in a physiological context and illuminate the occurrence of two pools of APP, one of which is linked to neuroendocrine cell activation.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2005.03243.x","type":"journal-article","created":{"date-parts":[[2005,7,29]],"date-time":"2005-07-29T16:25:17Z","timestamp":1122654317000},"page":"1015-1024","source":"Crossref","is-referenced-by-count":12,"title":["Biosynthesis and differential processing of two pools of amyloid\u2010\u03b2 precursor protein in a physiologically inducible neuroendocrine cell"],"prefix":"10.1111","volume":"94","author":[{"given":"Rob W. J.","family":"Collin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilhelmina H.","family":"Van Den Hurk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerard J. 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