{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T12:13:38Z","timestamp":1768479218022,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,7,5]],"date-time":"2005-07-05T00:00:00Z","timestamp":1120521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transcription co\u2010activators and histone acetyltransferases, p300 and cyclic AMP responsive element\u2010binding protein\u2010binding protein (CBP), participate in hypoxic activation of hypoxia\u2010inducible genes. Here, we show that exposure of PC12 and cells to 1\u201310% oxygen results in hyperphosphorylation of p300\/CBP. This response is fast, long lasting and specific for hypoxia, but not for hypoxia\u2010mimicking agents such as desferioxamine or Co<jats:sup>2+<\/jats:sup> ions. It is also cell\u2010type specific and occurs in pheochromocytoma PC12 cells and the carotid body of rats but not in hepatoblastoma cells. The p300 hyperphosphorylation specifically depends on the release of intracellular calcium from inositol 1,4,5\u2010triphosphate (IP<jats:sub>3<\/jats:sub>)\u2010sensitive stores. However, it is not inhibited by pharmacological inhibitors of any of the kinases traditionally known to be directly or indirectly calcium regulated. On the other hand, p300 hyperphosphorylation is inhibited by several different inhibitors of the glucose metabolic pathway from generation of NADH by glyceraldehyde 3\u2010phosphate dehydrogenase, through the transfer of NADH through the glycerol phosphate shuttle to ubiquinone and complex III of the mitochondrial respiratory chain. Inhibition of IP<jats:sub>3<\/jats:sub>\u2010sensitive calcium stores decreases generation of ATP, and this inhibition is significantly stronger in hypoxia than in normoxia. We propose that the NADH glycerol phosphate shuttle participates in generating a pool of ATP that serves either as a co\u2010factor or a modulator of the kinases involved in the phosphorylation of p300\/CBP during hypoxia.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2005.03293.x","type":"journal-article","created":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T16:49:26Z","timestamp":1133455766000},"page":"1288-1296","source":"Crossref","is-referenced-by-count":13,"title":["Hypoxia\u2010activated metabolic pathway stimulates phosphorylation of p300 and CBP in oxygen\u2010sensitive cells"],"prefix":"10.1111","volume":"94","author":[{"given":"Adriana","family":"Zakrzewska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phillip O.","family":"Schnell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin B.","family":"Striet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Hui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer R.","family":"Robbins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milan","family":"Petrovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Conforti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Gozal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc G.","family":"Wathelet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria F.","family":"Czyzyk\u2010Krzeska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,7,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/24190"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.23.12969"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.31.19834"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.1.64"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.132254299"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205432200"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001914200"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5382.1505"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/365855a0"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063518"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.7.4089"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1016\/S0021-9258(17)34160-1","article-title":"Biosynthesis Escherichia coli of sn\u2010glycerol 3\u2010phosphate, a precursor of phospholipids: palmitoyl\u2010CoA inhibition of the biosynthetic sn\u2010glycerol\u20103\u2010phosphate dehydrogenase","volume":"254","author":"Edgar J. 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