{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T19:54:14Z","timestamp":1760471654040,"version":"3.32.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T00:00:00Z","timestamp":1132531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The major isoforms of GABA<jats:sub><jats:sc>A<\/jats:sc><\/jats:sub>receptors are thought to be composed of two \u03b1, two \u03b2 and one \u03b3 subunit(s). GABA<jats:sub><jats:sc>A<\/jats:sc><\/jats:sub>receptors containing two \u03b2<jats:sub>1<\/jats:sub>subunits respond differently to the anticonvulsive compound loreclezole and the general anaesthetic etomidate than receptors containing two \u03b2<jats:sub>2<\/jats:sub>subunits. Receptors containing \u03b2<jats:sub>2<\/jats:sub>subunits show a much larger allosteric stimulation by these agents than those containing \u03b2<jats:sub>1<\/jats:sub>subunits. We were interested to know how receptors containing both \u03b2<jats:sub>1<\/jats:sub>and \u03b2<jats:sub>2<\/jats:sub>subunits, in different positions respond to loreclezole and etomidate. To answer this question, subunits were fused at the DNA level to form dimeric and trimeric subunits. Concatenated receptors (\u03b1<jats:sub>1<\/jats:sub>\u2010\u03b2<jats:sub>1<\/jats:sub>\u2010\u03b1<jats:sub>1<\/jats:sub>\/\u03b3<jats:sub>2<\/jats:sub>\u2010\u03b2<jats:sub>1<\/jats:sub>, \u03b1<jats:sub>1<\/jats:sub>\u2010\u03b2<jats:sub>2<\/jats:sub>\u2010\u03b1<jats:sub>1<\/jats:sub>\/\u03b3<jats:sub>2<\/jats:sub>\u2010\u03b2<jats:sub>1<\/jats:sub>, \u03b1<jats:sub>1<\/jats:sub>\u2010\u03b2<jats:sub>1<\/jats:sub>\u2010\u03b1<jats:sub>1<\/jats:sub>\/\u03b3<jats:sub>2<\/jats:sub>\u2010\u03b2<jats:sub>2<\/jats:sub>and \u03b1<jats:sub>1<\/jats:sub>\u2010\u03b2<jats:sub>2<\/jats:sub>\u2010\u03b1<jats:sub>1<\/jats:sub>\/\u03b3<jats:sub>2<\/jats:sub>\u2010\u03b2<jats:sub>2<\/jats:sub>) were expressed in Xenopus ooctyes and functionally compared in their response to the agonist GABA and to the positive allosteric modulators, loreclezole and etomidate. We have shown that (I) in the presence of both \u03b2<jats:sub>1<\/jats:sub>and \u03b2<jats:sub>2<\/jats:sub>subunits in the same pentamer (mixed receptors) direct gating by etomidate is similar to exclusively \u03b2<jats:sub>1<\/jats:sub>containing receptors; (II) In mixed receptors, stimulation by etomidate assumed characteristics intermediate to exclusively \u03b2<jats:sub>1<\/jats:sub>or \u03b2<jats:sub>2<\/jats:sub>containing receptors, but the values for the concentrations &lt; 10\u2003\u00b5<jats:sc>m<\/jats:sc>were always much closer to those observed in \u03b1<jats:sub>1<\/jats:sub>\u2010\u03b2<jats:sub>1<\/jats:sub>\u2010\u03b1<jats:sub>1<\/jats:sub>\/\u03b3<jats:sub>2<\/jats:sub>\u2010\u03b2<jats:sub>1<\/jats:sub>receptors; and (III) mixed receptors show no positional effects.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2005.03495.x","type":"journal-article","created":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T12:56:02Z","timestamp":1132577762000},"page":"1724-1731","source":"Crossref","is-referenced-by-count":15,"title":["Consequence of the presence of two different \u03b2 subunit isoforms in a GABA<sub>A<\/sub>receptor"],"prefix":"10.1111","volume":"95","author":[{"given":"Nathalie","family":"Boulineau","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Baur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Minier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erwin","family":"Sigel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-198607000-00004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199312000-00026"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0031-6997(24)01363-2","article-title":"International Union of Pharmacology. 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