{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T18:50:27Z","timestamp":1790016627873,"version":"4.0.1"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,4,25]],"date-time":"2006-04-25T00:00:00Z","timestamp":1145923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In non\u2010mammalian vertebrates, the pineal gland contains an endogenous circadian oscillator and serves as a photosensitive neuroendocrinal organ. To better understand the pineal phototransduction mechanism, we focused on the chicken putative blue\u2010light photoreceptive molecule, Cryptochrome4 (cCRY4). Here we report the molecular cloning of pineal\n                    <jats:italic>cCry4<\/jats:italic>\n                    cDNA, the\n                    <jats:italic>in vivo<\/jats:italic>\n                    expression of\n                    <jats:italic>cCry4<\/jats:italic>\n                    mRNA, and the detection of cCRY4 protein.\n                    <jats:italic>cCry4<\/jats:italic>\n                    is transcribed in a wide variety of chick tissues out of which the pineal gland and retina contain high levels of\n                    <jats:italic>cCry4<\/jats:italic>\n                    mRNA. In the pineal gland, under 12\u2003h light\u2003:\u200312\u2003h dark cycles, the levels of both\n                    <jats:italic>cCry4<\/jats:italic>\n                    mRNA and cCRY4 protein showed diurnal changes, and in cultured chick pineal cells, the\n                    <jats:italic>cCry4<\/jats:italic>\n                    mRNA level was not only up\u2010regulated by light but also controlled by circadian signals. Immunoblot analysis with a cCRY4\u2010specific antibody detected cCRY4 in a soluble fraction of the pineal lysate. Immunocytochemistry revealed that cCRY4 was expressed in many parenchymal cells and a limited number of stromal cells. These cCRY4 features strikingly contrast with those of the chick pineal photoreceptor pinopsin, suggesting a possible temporal and\/or spatial duplicity of the pineal photoreceptive system, the opsin\u2010 and CRY\u2010based mechanisms.\n                  <\/jats:p>","DOI":"10.1111\/j.1471-4159.2006.03826.x","type":"journal-article","created":{"date-parts":[[2006,4,25]],"date-time":"2006-04-25T13:14:39Z","timestamp":1145970879000},"page":"1155-1165","source":"Crossref","is-referenced-by-count":33,"title":["Molecular cloning, mRNA expression, and immunocytochemical localization of a putative blue\u2010light photoreceptor CRY4 in the chicken pineal gland"],"prefix":"10.1111","volume":"97","author":[{"given":"Yoko","family":"Kubo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Akiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitaka","family":"Fukada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiyuki","family":"Okano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,4,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-10-03681.1999"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000145238"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404851101"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00008-X"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1096973"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5415.760"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02874.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/282094a0"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.141090998"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2826.2003.00993.x"},{"key":"e_1_2_7_12_1","volume-title":"Phylip","author":"Felsenstein J.","year":"1996"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1079536"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01761"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a002836"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.11.022"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000109131"},{"key":"e_1_2_7_18_1","doi-asserted-by":"crossref","first-page":"7321","DOI":"10.1523\/JNEUROSCI.22-17-07321.2002","article-title":"Opsin\u2010G11\u2010mediated signaling pathway for photic entrainment of the chicken pineal circadian clock","volume":"22","author":"Kasahara T.","year":"2002","journal-title":"J. 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