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In the present study, we determined whether 5\u2010LOX is activated (translocation and production of leukotrienes) after oxygen\u2010glucose deprivation (OGD) in primary rat cortical neurons; if so, whether this activation is mediated by NMDA receptor. After OGD, 5\u2010LOX was translocated to the nuclear envelope as detected by immunoblotting, immunostaining and green fluorescent protein\u20105\u2010LOX transfection. 5\u2010LOX metabolites, cysteinyl\u2010leukotrienes (CysLTs) but not leukotriene B4, in the culture media were increased 0.5\u20131.5 h after recovery. Similarly, NMDA (100\u2003\u00b5<jats:sc>m<\/jats:sc>) also induced 5\u2010LOX translocation, and increased the production of CysLTs during 0.5\u20131 h NMDA exposure. Both OGD and NMDA reduced neuron viability. NMDA receptor antagonist MK\u2010801 inhibited almost all the responses to OGD and NMDA; whereas 5\u2010LOX activating protein inhibitor MK\u2010886 and 5\u2010LOX inhibitor caffeic acid inhibited the reduction of neuron viability and the production of CysLTs, but did not affect 5\u2010LOX translocation. From these results, we conclude that OGD can activate 5\u2010LOX in primary rat cortical neurons, and that this activation may be partly mediated via activating NMDA receptor.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2006.03828.x","type":"journal-article","created":{"date-parts":[[2006,4,5]],"date-time":"2006-04-05T12:09:48Z","timestamp":1144238988000},"page":"992-1004","source":"Crossref","is-referenced-by-count":35,"title":["Activation of 5\u2010lipoxygenase after oxygen\u2010glucose deprivation is partly mediated via NMDA receptor in rat cortical neurons"],"prefix":"10.1111","volume":"97","author":[{"given":"Qiu\u2010Fu","family":"Ge","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Er\u2010Qing","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Ping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Jia","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen\u2010Qiu","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010Hong","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,4,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000028184.80776.58"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)02491-X"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/hs1001.096057"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/37635"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.4049\/jimmunol.162.3.1669","article-title":"Decreased leukotriene C4 synthesis accompanies adherence\u2010dependent nuclear import of 5\u2010lipoxygenase in human blood eosinophils","volume":"162","author":"Brock T. 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