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The ability of the neurokinin\u20101 receptor agonist [Sar<jats:sup>9<\/jats:sup>,Met(O<jats:sub>2<\/jats:sub>)<jats:sup>11<\/jats:sup>]\u2010substance P, nerve growth factor and brain derived neurotrophic factor to modulate positively preprotachykinin\u2010A gene promoter construct activity, as indicated by <jats:italic>de novo<\/jats:italic> enhanced green fluorescent protein expression, was determined. Treatment of organotypic slices with [Sar<jats:sup>9<\/jats:sup>, Met(O<jats:sub>2<\/jats:sub>)<jats:sup>11<\/jats:sup>]\u2010substance P (10\u2003\u03bc<jats:sc>m<\/jats:sc>, P\u2003&lt;\u20030.05), nerve growth factor (200\u2003ng\/mL, P\u2003&lt;\u20030.001) or brain derived neurotrophic factor (200\u2003ng\/mL, P\u2003&lt;\u20030.02) significantly increased the proportion of cytomegaloviral promoter\u2010DsRed transfected cells (used to visualise total transfected cells) that co\u2010expressed enhanced green fluorescent protein. The distribution of enhanced green fluorescent protein\/DsRed\u2010positive neurones across spinal laminae was broadly in line with the known distribution of spinal Trk and neurokinin\u20101 receptors. These data suggest a modulated activity of the preprotachykinin\u2010A gene promoter in spinal neurones <jats:italic>in vitro<\/jats:italic> by substance P and\/or neurotrophins. The functional consequences of such transcriptional changes within central peptidergic circuitry and their relevance to chronic pain are considered.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2006.03910.x","type":"journal-article","created":{"date-parts":[[2006,5,26]],"date-time":"2006-05-26T13:50:47Z","timestamp":1148651447000},"page":"690-699","source":"Crossref","is-referenced-by-count":4,"title":["Neurotrophin\u2010induced preprotachykinin\u2010A gene promoter modulation in organotypic rat spinal cord culture"],"prefix":"10.1111","volume":"98","author":[{"given":"Kathryn J.","family":"Hilton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan N.","family":"Bateson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne E.","family":"King","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(95)00343-H"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0295-168"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.21.9970"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890060305"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0953-816x.2001.01656.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(97)00067-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199503000-00005"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903560302"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(92)90237-V"},{"key":"e_1_2_7_11_1","first-page":"150","article-title":"Upregulation, release and axonal transport of substance P and calcitonin gene\u2010related peptide in adjuvant inflammation and regulatory function of nerve growth factor. 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