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The present study compares the ability of potential intercellular mediators of nitrosative damage including S\u2010nitrosoglutathione (GSNO), S\u2010nitrosocysteine and N<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub> to induce protein S\u2010nitros(yl)ation in the spinal cord, a CNS region that is commonly affected in multiple sclerosis and experimental allergic encephalomyelitis. The results clearly demonstrate that while all three NO\u2010donors cause S\u2010nitrosation of proteins in cell\u2010free systems, only GSNO is a viable S\u2010nitrosating agent in rat spinal cord slices. Generation of PrSNOs with GSNO occurs by S\u2010transnitrosation as the process was not inhibited by either the NO\u2010scavenger rutin or the N<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>\u2010scavenger azide. Contrary to other cell types, nerve cells incorporate intact GSNO and neither functional <jats:sc>l<\/jats:sc>\u2010amino acid transporters nor cell\u2010surface thiols are required. We also found that there is a restricted number of proteins available for S\u2010nitrosation, even at high, non\u2010physiological concentrations of GSNO. These proteins are highly concentrated in mitochondria and mitochondria\u2010rich subcellular compartments. This study is relevant to those CNS disorders characterized by excessive nitric oxide production.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2006.04180.x","type":"journal-article","created":{"date-parts":[[2006,10,2]],"date-time":"2006-10-02T11:54:29Z","timestamp":1159790069000},"page":"1299-1310","source":"Crossref","is-referenced-by-count":15,"title":["Extracellular S\u2010nitrosoglutathione, but not S\u2010nitrosocysteine or N<sub>2<\/sub>O<sub>3<\/sub>, mediates protein S\u2010nitrosation in rat spinal cord slices*"],"prefix":"10.1111","volume":"99","author":[{"given":"Jorge M.","family":"Romero","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar A.","family":"Bizzozero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/10715760000301001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(85)90723-4"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1995.1231"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2000.081"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.26.12041"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.5.C1424"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.126.4.1116"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)80353-9"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"7886","DOI":"10.1016\/S0021-9258(18)42596-3","article-title":"Characterization of proteolipid protein fatty acylesterase from rat brain myelin","volume":"267","author":"Bizzozero O. 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