{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T21:57:46Z","timestamp":1762898266530},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,1,22]],"date-time":"2007-01-22T00:00:00Z","timestamp":1169424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Machado\u2013Joseph disease (MJD) is an autosomal dominant neurodegenerative disorder caused by an expansion of the polyglutamine tract near the C\u2010terminus of the <jats:italic>MJD\u20101<\/jats:italic> gene product, ataxin\u20103. Ataxin\u20103 is degraded by the proteasome. However, the precise mechanism of ataxin\u20103 degradation remains to be elucidated. In this study, we show direct links between ataxin\u20103 and the proteasome. p45, an ATPase subunit of the 19S proteasome, interacts with ataxin\u20103 <jats:italic>in vitro<\/jats:italic> and stimulates the degradation of ataxin\u20103 in an <jats:italic>in vitro<\/jats:italic> reconstituted degradation assay system. The effect of p45 on ataxin\u20103 degradation is blocked by MG132, a proteasome inhibitor. In N2a or 293 cells, overexpression of p45 strikingly enhances the clearance of both normal and expanded ataxin\u20103, but not alpha synuclein or SOD1, implying a functional specificity of p45 in this proteolytic process. The N\u2010terminus of ataxin\u20103, which serves as a recognition site by p45, is necessary for the proteolytic process of ataxin\u20103. We also show that other three ATPases of the 19S proteasome, MSS1, p48, and p56 have no effect on ataxin\u20103 degradation. These data provide evidence that p45 plays an important role in regulating ataxin\u20103 degradation by the proteasome.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2007.04460.x","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T02:34:16Z","timestamp":1171506856000},"page":"1651-1661","source":"Crossref","is-referenced-by-count":16,"title":["p45, an ATPase subunit of the 19S proteasome, targets the polyglutamine disease protein ataxin\u20103 to the proteasome"],"prefix":"10.1111","volume":"101","author":[{"given":"Hongfeng","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nali","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erkang","family":"Fei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mian","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuyuki","family":"Nukina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiangning","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guanghui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(03)00053-4"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M506084200"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb815"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407252102"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg344"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-23-10338.1999"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/8.4.673"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409719102"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.07.131"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/502"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.18.6469-6483.2003"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302596200"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.4.1970"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006928316738"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2734-04.2004"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.19.110701.154617"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0437870100"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103736200"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.13.2009"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1194-221"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/1521654032000114339"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/35056572"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205259200"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506344102"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19981201)71:3<429::AID-JCB11>3.0.CO;2-P"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600081"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.14.2197"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313097200"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1587"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0501732102"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410400514"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.11.6020"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410410408"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80943-5"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.17.4909"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg297"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.10101"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb790"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3480281"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm780"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00723"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.06.014"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6484"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.12.1795"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.02.030"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/35056563"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.10.5461"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1471-4159.2007.04460.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1471-4159.2007.04460.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T15:29:09Z","timestamp":1697038149000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1471-4159.2007.04460.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1,22]]},"references-count":47,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2007,6]]}},"alternative-id":["10.1111\/j.1471-4159.2007.04460.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1471-4159.2007.04460.x","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1,22]]}}}