{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T03:38:21Z","timestamp":1773459501913,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T00:00:00Z","timestamp":1169510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have assessed the expression, molecular identification and functional role of Na<jats:sup>+<\/jats:sup> channels (Na<jats:sub>v<\/jats:sub>) in carotid bodies (CB) obtained from normoxic and chronically hypoxic adult rats. Veratridine evoked release of catecholamines (CA) from an <jats:italic>in vitro<\/jats:italic> preparation of intact CBs obtained from normoxic animals, the response being Ca<jats:sup>2+<\/jats:sup> and Na<jats:sup>+<\/jats:sup>\u2010dependent and sensitive to tetrodotoxin (TTX). TTX inhibited by 25\u201350% the CA release response evoked by graded hypoxia. Immunoblot assays demonstrated the presence of Na<jats:sub>v<\/jats:sub>\u03b1\u2010subunit (<jats:italic>c.<\/jats:italic> 220\u2003kDa) in crude homogenates from rat CBs, being evident an up\u2010regulation (60%) of this protein in the CBs obtained from chronically hypoxic rats (10% O<jats:sub>2<\/jats:sub>; 7\u2003days). This up\u2010regulation was accompanied by an enhanced TTX\u2010sensitive release response to veratridine, and by an enhanced ventilatory response to acute hypoxic stimuli. RT\u2010PCR studies demonstrated the expression of mRNA for Na<jats:sub>v<\/jats:sub>1.1, Na<jats:sub>v<\/jats:sub>1.2, Na<jats:sub>v<\/jats:sub>1.3, Na<jats:sub>v<\/jats:sub>1.6 and Na<jats:sub>v<\/jats:sub>1.7 isoforms. At least three isoforms, Na<jats:sub>v<\/jats:sub>1.1, Na<jats:sub>v<\/jats:sub>1.3 and Na<jats:sub>v<\/jats:sub>1.6 co\u2010localized with tyrosine hydroxylase in all chemoreceptor cells. RT\u2010PCR and immunocytochemistry indicated that Na<jats:sub>v<\/jats:sub>1.1 isoform was up\u2010regulated by chronic hypoxia in chemoreceptor cells. We conclude that Na<jats:sub>v<\/jats:sub> up\u2010regulation represents an adaptive mechanism to increase chemoreceptor sensitivity during acclimatization to sustained hypoxia as evidenced by enhanced ventilatory responses to acute hypoxic tests.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2007.04465.x","type":"journal-article","created":{"date-parts":[[2007,3,9]],"date-time":"2007-03-09T17:26:13Z","timestamp":1173461173000},"page":"231-245","source":"Crossref","is-referenced-by-count":30,"title":["Molecular identification and functional role of voltage\u2010gated sodium channels in rat carotid body chemoreceptor cells. Regulation of expression by chronic hypoxia <i>in vivo<\/i>"],"prefix":"10.1111","volume":"102","author":[{"given":"Ana I.","family":"Caceres","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Obeso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constancio","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asuncion","family":"Rocher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.57.030195.002035"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01156.2004"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000109455"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5687(00)00131-6"},{"key":"e_1_2_7_6_1","first-page":"617","volume-title":"Regulation of Breathing","author":"Bisgard G. 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