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Here we found that PACAP\u201038 elevated cytosolic [Ca<jats:sup>2+<\/jats:sup>] ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) in the oxygen sensing type I cells but not the glial\u2010like type II (sustentacular) cells of the rat carotid body. This action of PACAP could not be mimicked by vasoactive intestinal peptide but was abolished by PACAP 6\u201038, implicating the involvement of PAC<jats:sub>1<\/jats:sub>receptors. H89, a protein kinase A (PKA) inhibitor attenuated the PACAP response. Simultaneous measurement of membrane potential and [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>showed that the PACAP\u2010mediated [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>rise was accompanied by depolarization and action potential firing. Ni<jats:sup>2+<\/jats:sup>, a blocker of voltage\u2010gated Ca<jats:sup>2+<\/jats:sup>channels (VGCC) or the removal of extracellular Ca<jats:sup>2+<\/jats:sup>reversibly inhibited the PACAP\u2010mediated [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>rise. In the presence of tetraethylammonium (TEA) and 4\u2010aminopyridine (4\u2010AP), PACAP reduced a background K<jats:sup>+<\/jats:sup>current. Anandamide, a blocker of TWIK\u2010related acid\u2010sensitive K<jats:sup>+<\/jats:sup>(TASK)\u2010like K<jats:sup>+<\/jats:sup>channel, occluded the inhibitory action of PACAP on K<jats:sup>+<\/jats:sup>current. We conclude that PACAP, acting via the PAC<jats:sub>1<\/jats:sub>receptors coupled PKA pathway inhibits a TASK\u2010like K<jats:sup>+<\/jats:sup>current and causes depolarization and VGCC activation. This stimulatory action of PACAP in carotid type I cells can partly account for the role of PACAP in respiratory disorders.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2007.04468.x","type":"journal-article","created":{"date-parts":[[2007,3,9]],"date-time":"2007-03-09T17:26:13Z","timestamp":1173461173000},"page":"1284-1293","source":"Crossref","is-referenced-by-count":21,"title":["Pituitary adenylate cyclase\u2010activating polypeptide (PACAP) stimulates the oxygen sensing type I (glomus) cells of rat carotid bodies via reduction of a background TASK\u2010like K<sup>+<\/sup>current"],"prefix":"10.1111","volume":"101","author":[{"given":"Fenglian","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick W.","family":"Tse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Tse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,1,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240000368"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-19-07353.2000"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0079-6107(82)90011-6"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1997.sp021890"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020239"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020143"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(01)00556-1"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.052514"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1997.sp021861"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404743200"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0304-3940(02)01316-2"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4179(97)90087-1"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1152\/jn.1993.70.4.1378","article-title":"L\u2010type calcium channels in type I cells of the rat carotid body","volume":"70","author":"Fieber L. 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