{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T14:28:38Z","timestamp":1759588118376},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T00:00:00Z","timestamp":1172793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We recently reported a diurnal and norepinephrine (NE) \u2010induced expression of mitogen\u2010activated protein kinase (MAPK) phosphatase\u20101 (MKP\u20101) in the rat pineal gland and postulated that this MKP\u20101 expression might impact adrenergic\u2010regulated arylalkylamine\u2010<jats:italic>N<\/jats:italic>\u2010acetyltransferase (AA\u2010NAT) activity via modulation of MAPKs. In this study, we investigated the effect of depletion of MKP\u20101 expression by using doxorubicin, a topoisomerase inhibitor that suppresses the expression of MKP\u20101 in other cell types and small interfering RNA targeted against <jats:italic>Mkp1<\/jats:italic> in NE\u2010stimulated pinealocytes. We found that both treatments were effective in inhibiting NE induction of MKP\u20101 expression. Moreover, both treatments also resulted in a prolonged activation of p42\/44MAPK and an increase in AA\u2010NAT induction by NE. In contrast, treatment of pinealocytes with PD98059, an inhibitor of MAPK kinase, reduced NE\u2010stimulated AA\u2010NAT activity. Interestingly, suppressing MKP\u20101 expression had no effect on the time profile of NE\u2010stimulated p38MAPK activation. These results indicate that MKP\u20101 modulates the profile of AA\u2010NAT activity by selectively shaping the activation profile of p42\/44MAPK but not that of p38MAPK.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2007.04557.x","type":"journal-article","created":{"date-parts":[[2007,5,2]],"date-time":"2007-05-02T15:43:27Z","timestamp":1178120607000},"page":"1685-1693","source":"Crossref","is-referenced-by-count":11,"title":["Mitogen\u2010activated protein kinase phosphatase\u20101 (MKP\u20101) preferentially dephosphorylates p42\/44MAPK but not p38MAPK in rat pinealocytes"],"prefix":"10.1111","volume":"101","author":[{"given":"Donald M.","family":"Price","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin T.","family":"Wloka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constance L.","family":"Chik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony K.","family":"Ho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1096\/fasebj.14.1.6","article-title":"Dual specificity phosphatases: a gene family for control of MAP kinase function","volume":"14","author":"Camps M.","year":"1999","journal-title":"FASEB J."},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.68031078.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-0864"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.13.7161"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.27.16917"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.141.12.4496"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(03)00260-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-0215"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2006.03.022"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072682"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(99)00075-7"},{"key":"e_1_2_7_13_1","first-page":"307","article-title":"The melatonin rhythm\u2010generating enzyme: molecular regulation of serotonin N\u2010acetyltransferase in the pineal gland","volume":"52","author":"Klein D.C.","year":"1997","journal-title":"Recent Prog. 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