{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T02:51:56Z","timestamp":1774407116827,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,7,17]],"date-time":"2007-07-17T00:00:00Z","timestamp":1184630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previously, we have shown that ethanol\u2010induced apoptosis in cultured neurons is accompanied by changes in cellular lipid profiles. In the present study, the effects of ethanol on brain lipid metabolism were studied using 7\u2010day\u2010old C57BL\/6ByJ mice, which display apoptotic neurodegeneration upon exposure to ethanol. The brain lipids were extracted 4\u201324\u2003h after the ethanol or saline treatment, and analyzed by TLC. We found that the levels of triglyceride, cholesterol ester, ceramide, and <jats:italic>N<\/jats:italic>\u2010acylphosphatidylethanolamine increased significantly in the brains of ethanol\u2010treated mice compared to those of saline\u2010treated mice. Concomitantly, ethanol reduced Thr172 phosphorylation of AMP\u2010activated protein kinase (AMPK) \u03b1 subunits. Ethanol also reduced phosphorylation of acetyl\u2010CoA carboxylase, a substrate of AMPK and a lipogenic enzyme known to be activated by dephosphorylation. In contrast, lipid profiles of 19\u2010day\u2010old mouse brains, which scarcely manifested neurodegeneration upon ethanol exposure, were not significantly affected by ethanol. Also, the basal levels of Thr172\u2010phosphorylated AMPK \u03b1 were lower in these brains than in 7\u2010day\u2010old mouse brains, and no detectable changes in the phosphorylation status were observed by ethanol treatment. Our findings indicate that the ethanol\u2010induced apoptotic neurodegeneration observed in mice during restricted developmental periods is accompanied by alterations in both the lipid content and the activity of AMPK in the brain.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2007.04836.x","type":"journal-article","created":{"date-parts":[[2007,8,7]],"date-time":"2007-08-07T12:29:36Z","timestamp":1186489776000},"page":"1208-1218","source":"Crossref","is-referenced-by-count":34,"title":["Ethanol alters lipid profiles and phosphorylation status of AMP\u2010activated protein kinase in the neonatal mouse brain"],"prefix":"10.1111","volume":"103","author":[{"given":"Mariko","family":"Saito","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goutam","family":"Chakraborty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui\u2010Fen","family":"Mao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ray","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas B.","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Csaba","family":"Vadasz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuo","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,7,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C300557200"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000120374.59826.1b"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02089-0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2005.08.040"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02341.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2004.07.014"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1385\/JMN:17:1:45"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(20000415)60:2<141::AID-JNR2>3.0.CO;2-5"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2005.12.065"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.0750861.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00006.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.108944"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.44.27879"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devbrainres.2003.08.005"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C500398200"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.08.043"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5455.1056"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-7075-3-15"},{"key":"e_1_2_7_20_1","first-page":"139","volume-title":"Methods in Enzymology","author":"Ledeen R. W.","year":"1982"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1385\/MN:22:1-3:041"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02440"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S0022-2275(20)32456-1","article-title":"Accumulation of N\u2010acyl\u2010ethanolamine phospholipids in rat brains during post\u2010decapitative ischemia: a 31p NMR study","volume":"40","author":"Moesgaard B.","year":"1999","journal-title":"J. Lipid Res."},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3380783"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90484-6"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(02)00279-1"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.02130.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(86)90164-1"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490180208"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.alc.0000175011.22307.61"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2007.00351.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2006.01482.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.5.2498"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.2.611"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.5.2327"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1053\/sp.2000.6361"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00056.2004"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2005.12.015"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2005.04.014"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.4.1784"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1471-4159.2007.04836.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1471-4159.2007.04836.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T22:14:05Z","timestamp":1696976045000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1471-4159.2007.04836.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7,17]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,11]]}},"alternative-id":["10.1111\/j.1471-4159.2007.04836.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1471-4159.2007.04836.x","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7,17]]}}}