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Minocycline\u2019s mechanisms of action as a neuroprotective and anti\u2010allodynic agent are unknown. In response to injury, microglia become activated, proliferate, and migrate. Resting microglia express voltage\u2010dependent inward K<jats:sup>+<\/jats:sup> currents and blocking Kv1.3 channels has been shown to inhibit microglial\u2010mediated neuronal death. We investigated the effect of minocycline on the expression of Kv channels, cell motility, and \u03b2\u2010integrin expression using primary rat cortical microglia, transwell assays, and by flow cytometry. Minocycline significantly reduced microglial migration to cellular debris, astrocyte\u2010conditioned medium, ADP, and algesic mediators and significantly reduced the expression of CD29 (\u03b2<jats:sub>1<\/jats:sub>\u2010integrin) but not CD18 (\u03b2<jats:sub>2<\/jats:sub>\u2010integrin). Minocycline reduced the effect of extracellular potassium and later decreased microglial Kv1.3 expression. In summary, we uncovered a novel effect of minocycline that demonstrates this agent decreases microglial \u03b2<jats:sub>1<\/jats:sub>\u2010integrin expression, which leads to inhibition of motility. We propose an <jats:italic>in vivo<\/jats:italic> model whereby reduced microglial trafficking to injured neurons following nerve injury decreases the release of proinflammatory mediators into the synaptic milieu, preventing neuronal sensitization, the pathological correlate to chronic pain.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2007.04889.x","type":"journal-article","created":{"date-parts":[[2007,9,17]],"date-time":"2007-09-17T05:41:03Z","timestamp":1190007663000},"page":"2035-2046","source":"Crossref","is-referenced-by-count":77,"title":["Minocycline decreases <i>in vitro<\/i> microglial motility, \u03b2<sub>1<\/sub>\u2010integrin, and Kv1.3 channel expression"],"prefix":"10.1111","volume":"103","author":[{"given":"Nancy","family":"Nutile\u2010McMenemy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arye","family":"Elfenbein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joyce 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